High incidence of intact or fragmented immunoglobulin in urine of patients with multiple myeloma

In this prospective study we determined the incidence of intact/fragmented immunoglobulin and Bence Jones protein in urine immunofixation using Sebia reagents and HydrasysTM 2 apparatus and compared the results to concentrations of serum free light chains (FLC) assessed using Siemens BNTM II nephelo...

Full description

Saved in:
Bibliographic Details
Published in:Leukemia & lymphoma 2015-12, Vol.56 (12), p.3348-3356
Main Authors: Kraj, Maria, Kruk, Barbara, Lech-Marańda, Ewa, Warzocha, Krzysztof, Prochorec-Sobieszek, Monika
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Female
Follow-Up Studies
Humans
Immunoglobulin Fragments - blood
Immunoglobulin Fragments - urine
Immunoglobulin Isotypes - blood
Immunoglobulin Isotypes - urine
Immunoglobulin Light Chains - blood
Immunoglobulin Light Chains - urine
Immunoglobulins - blood
Immunoglobulins - urine
Incidence
Kidney Function Tests
Male
Middle Aged
Multiple Myeloma - complications
Multiple Myeloma - diagnosis
Multiple Myeloma - epidemiology
Multiple Myeloma - mortality
Multiple Myeloma - urine
Neoplasm Staging
Proteinuria - epidemiology
Proteinuria - etiology
Survival Analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this prospective study we determined the incidence of intact/fragmented immunoglobulin and Bence Jones protein in urine immunofixation using Sebia reagents and HydrasysTM 2 apparatus and compared the results to concentrations of serum free light chains (FLC) assessed using Siemens BNTM II nephelometer and the immunoassay Freelite (Binding Site) in 289 patients with multiple myeloma at diagnosis. It was found that in one third of IgG, IgA and IgD myeloma patients, intact/fragmented immunoglobulin can be detected in urine and is connected with impaired renal function and reduced survival. Urine immunofixation detects monoclonal protein (FLC and intact/fragmented immunoglobulin) in 66-79% of IgG and IgA myeloma patients while serum FLC immunoassay detect it in 82-94% of IgG and IgA myeloma patients. However, the latter method is inadequate for detection of intact/fragmented immunoglobulin in urine. Serum FLC immunoassay and urine immunofixation are complementary methods in diagnosing and monitoring monoclonal protein in patients with myeloma.
ISSN:1042-8194
1029-2403
DOI:10.3109/10428194.2015.1037753