Comparative characterization of nucleotides, nucleosides and nucleobases in Abelmoschus manihot roots, stems, leaves and flowers during different growth periods by UPLC-TQ-MS/MS

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2015-12, Vol.1006, p.130-137
• Main Authors: Du, Le-yue, Qian, Da-wei, Jiang, Shu, Shang, Er-xin, Guo, Jian-ming, Liu, Pei, Su, Shu-lan, Duan, Jin-ao, Zhao, Min
• Format: Article
• Language: English
• Subjects: Abelmoschus - chemistry; Abelmoschus - growth & development; Abelmoschus manihot; Chromatography, High Pressure Liquid - methods; Growth periods; Limit of Detection; Linear Models; Nucleobases; Nucleosides; Nucleosides - analysis; Nucleotides; Nucleotides - analysis; Plant Components, Aerial - chemistry; Plant Extracts - chemistry; Plant Roots - chemistry; Reproducibility of Results; Tandem Mass Spectrometry - methods; UPLC-TQ-MS/MS
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2015.10.021

•A. manihot is an annual herbal plant of folk medicine and widely distributes in many countries.•This method could be utilised for the quality control of the A. manihot and other herbal medicines.•The distribution and contents of 12 analytes in four parts were arranged as leaf>flower>stem>root.•The leaves and flowers can be developed as nutraceutical and bioactive products. Nucleotides, nucleosides and nucleobases have been proven as important bioactive compounds related to many physiological processes. Abelmoschus manihot (L.) Medicus from the family of Malvaceae is an annual herbal plant of folk medicine widely distributed in Oceania and Asia. However, up to now, no detailed information could be available for the types and contents of nucleotides, nucleosides and nucleobases contained in A. manihot roots, stems, leaves as well as the flowers. In the present study, an UPLC-TQ-MS/MS method was established for detection of the twelve nucleotides, nucleosides and nucleobases. The validated method was successfully applied to identify the 12 analytes in different parts of A. manihot harvested at ten growth periods. 2′-deoxyinosine was not detected in all of the A. manihot samples. The data demonstrated that the distribution and concentration of the 12 compounds in A. manihot four parts were arranged in a decreasing order as leaf>flower>stem>root. Based on the results, the leaves and flowers of A. manihot could be developed as health products possessed nutraceutical and bioactive properties in the future. This method might also be utilized for the quality control of the A. manihot leaves and other herbal medicines being rich in nucleotides, nucleosides and nulecobases.