Optimization of diacylglycerol production by glycerolysis of fish oil catalyzed by Lipozyme TL IM with Tween 65

The diacylglycerols (DAG) are emulsifiers with high added value used as functional additives in food, medicine, and cosmetic industries. In glycerolysis of oils for the production of DAG, the immiscibility between the substrates (glycerol and oil phases) has to be overcome, for example, by the addit...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2015-12, Vol.38 (12), p.2379-2388
Main Authors: Monte Blanco, S. F. M, Santos, J. S, Feltes, M. M. C, Dors, G, Licodiedoff, S, Lerin, L. A, de Oliveira, D, Ninow, J. L, Furigo, A., Jr
Format: Article
Language:English
Subjects:
biocatalysts
Bioengineering
Biotechnology
Catalysis
Chemistry
Chemistry and Materials Science
diacylglycerols
Diglycerides - biosynthesis
docosahexaenoic acid
eicosapentaenoic acid
emulsifiers
Environmental Engineering/Biotechnology
Fish oils
Fish Oils - metabolism
food additives
Food Science
glycerol
Glycerol - metabolism
Hydrolysis
Immiscibility
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
industry
Lipase - metabolism
medicine
Oils & fats
Original Paper
Surface-Active Agents - chemistry
Surfactants
temperature
value added
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Summary:The diacylglycerols (DAG) are emulsifiers with high added value used as functional additives in food, medicine, and cosmetic industries. In glycerolysis of oils for the production of DAG, the immiscibility between the substrates (glycerol and oil phases) has to be overcome, for example, by the addition of a food grade surfactant like Tween 65. The main objective of this work was to optimize the process conditions for obtaining diacylglycerols rich in the omega-3 eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, through the enzymatic glycerolysis of fish oil, in systems with Tween 65 and without this surfactant, using Lipozyme TL® IM as biocatalyst. The experiments were performed according to predetermined conditions varying the temperature, enzyme load, the oil to glycerol molar ratio and, when added, the surfactant load. After 6 h of reaction, it was possible to produce up to 20.76 and 13.76 % of diacylglycerols from fish oil in the reactions with and without Tween 65, respectively.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-015-1473-9