Rapid detection of genetically diverse tomato black ring virus isolates using reverse transcription loop-mediated isothermal amplification

A reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) has been developed for detection of tomato black ring virus (TBRV) isolates collected from different hosts. One-step RT-LAMP was performed with a set of four primers, the design of which was based on the coat protein gene...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2015-12, Vol.160 (12), p.3075-3078
Main Authors: Hasiów-Jaroszewska, Beata, Budzyńska, Daria, Borodynko, Natasza, Pospieszny, Henryk
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
DNA Primers - genetics
Genetic Variation
Genomes
Infectious Diseases
Lycopersicon esculentum - virology
Medical Microbiology
Nepovirus - classification
Nepovirus - genetics
Nepovirus - isolation & purification
Nucleic Acid Amplification Techniques - methods
Plant Diseases - virology
Residuals
Reverse Transcription
RNA polymerase
Sensitivity and Specificity
Tomato black ring virus
Virology
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) has been developed for detection of tomato black ring virus (TBRV) isolates collected from different hosts. One-step RT-LAMP was performed with a set of four primers, the design of which was based on the coat protein gene. Results of RT-LAMP were visualized by direct staining of products with fluorescent dyes, agarose gel electrophoresis, and analysis of amplification curves. The sensitivity of RT-LAMP was 100-fold greater than that of RT-PCR. The RT-LAMP assay developed here is a useful and practical method for diagnosis of TBRV.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-015-2586-9