Classical swine fever virus marker vaccine strain CP7_E2alf: genetic stability in vitro and in vivo

Recently, CP7_E2alf (Suvaxyn®CSF Marker), a live marker vaccine against classical swine fever virus, was licensed through the European Medicines Agency. For application of such a genetically engineered virus under field conditions, knowledge about its genetic stability is essential. Here, we report...

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Bibliographic Details
Published in:Archives of virology 2015-12, Vol.160 (12), p.3121-3125
Main Authors: Goller, Katja V, Dräger, Carolin, Höper, Dirk, Beer, Martin, Blome, Sandra
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brief Report
Cell culture
Classical Swine Fever - prevention & control
Classical Swine Fever - virology
Classical swine fever virus
Classical swine fever virus - genetics
Classical swine fever virus - immunology
Fever
genetic engineering
genetic stability
Genomes
Genomic Instability
Hogs
Immunization
Infectious Diseases
Medical Microbiology
mutation
sequence analysis
Software
Swine
Trade restrictions
Vaccines
Vaccines, Marker - genetics
Vaccines, Marker - immunology
Viral Vaccines - genetics
Viral Vaccines - immunology
Virology
Viruses
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Summary:Recently, CP7_E2alf (Suvaxyn®CSF Marker), a live marker vaccine against classical swine fever virus, was licensed through the European Medicines Agency. For application of such a genetically engineered virus under field conditions, knowledge about its genetic stability is essential. Here, we report on stability studies that were conducted to assess and compare the mutation rate of CP7_E2alf in vitro and in vivo. Sequence analyses upon passaging confirmed the high stability of CP7_E2alf, and no recombination events were observed in the experimental setup. The data obtained in this study confirm the genetic stability of CP7_E2alf as an important safety component.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-015-2611-z