Molecular identification and expression of the Foxl2 gene during gonadal sex differentiation in northern snakehead Channa argus

• Published in: Fish physiology and biochemistry 2015-12, Vol.41 (6), p.1419-1433
• Main Authors: Wang, Dan-Dan, Zhang, Gui-Rong, Wei, Kai-Jian, Ji, Wei, Gardner, Jonathan P. A, Yang, Rui-Bin, Chen, Kun-Ci
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Amino acids; Androgens; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Biomedical and Life Sciences; brain; Cell differentiation; Channa argus; Cloning, Molecular; complementary DNA; Female; Females; fish; Fish Proteins - genetics; Fish Proteins - metabolism; Fisheries; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Freshwater & Marine Ecology; Gene Expression Regulation, Developmental; Genes; Gonads - physiology; granulosa cells; Granulosa Cells - physiology; Histology; Life Sciences; Male; males; Molecular Sequence Data; Morphology; ontogeny; oocytes; open reading frames; Ovaries; Ovary - physiology; Perciformes - genetics; Perciformes - physiology; reverse transcriptase polymerase chain reaction; Sex Differentiation - genetics; sexual development; testes; Testis - physiology; Transcription factors; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-015-0096-z

Channa argus is one of the most commercially important fish species in China. Studies show that males of C. argus grow faster than females at the same age. In order to explore the sex differentiation mechanism of C. argus, we isolated the full length of the sex-related gene Foxl2 cDNA and analysed its expression patterns during gonadal sex differentiation. Alignment of known Foxl2 amino acid sequences from vertebrates confirmed the conservation of the Foxl2 open reading frame, especially the forkhead domain and C-terminal region. Quantitative RT-PCR revealed that Foxl2 is predominantly expressed in brain, pituitary, gill and ovary, with its highest level in ovary but low levels in testis and other tissues, reflecting a potential role for Foxl2 in the brain–pituitary–gonad axis in C. argus. Our ontogenetic stage data showed that C. argus Foxl2 expression was significantly upregulated from 1 to 11 days posthatching (dph) and that the initiation of expression preceded the first anatomical ovarian differentiation (27 dph), suggesting that Foxl2 might play a potential role in early gonadal sex differentiation in C. argus. In addition, the Foxl2 protein was primarily located in granulosa cells surrounding the oocytes of mature C. argus, implying that Foxl2 may have a basic function in granulosa cell differentiation and the maintenance of oocytes.