Lysobacter hymeniacidonis sp. nov., Isolated from a Crude Oil-Contaminated Marine Sponge

• Published in: Journal of Ocean University of China 2015-12, Vol.14 (6), p.1019-1024
• Main Authors: Xin, Yanjuan, Qu, Junge, Xu, Junyi, Wu, Peichun, Cao, Xupeng, Xue, Song
• Format: Article
• Language: English
• Subjects: analysis; Aquatic life; Contamination; Crude oil; Deoxyribonucleic acid; DNA; Earth and Environmental Science; Earth Sciences; hymeniacidonis; Lipids; Lysobacter; Marine; Marine pollution; Meteorology; Oceanography; Oil pollution; phylogenetic; Phylogenetics; Sodium chloride; sp.nov; Taxonomy
• ISSN: 1672-5182; 1993-5021; 1672-5174
• DOI: 10.1007/s11802-015-2789-4

An aerobic, Gram-negative bacterium, strain 2-5~T, was isolated from a crude oil-contaminated marine sponge collected near Dalian Bay, China, and subjected to a polyphasic taxonomic investigation. Cells of strain 2-5~T were non-spore forming, non-motile, rods 0.2–0.3 μm wide and 1.1–1.2μm long. Strain 2-5~T grew well on nutrient agar, ~TSA, R2 A agar and LB agar. Colonies of strain 2-5~T on LB agar were circular, smooth with entire margins, non-transparent and pale yellow after 3 d of incubation at 30℃. Growth of strain 2-5~T occurred in LN medium with 0–6% Na Cl; no growth occurred in the presence of 8.0% NaCl. Strain 2-5~T grew at 15–42℃ and at pH 6.0–8.0. Comparative 16 S r RNA gene sequence analysis showed that strain 2-5~T clustered with the species of the genus Lysobacter. Its closet neighbors were the type strains of Lysobacter concretionis KC~TC 12205~T（97% similarity）, Lysobacter arseniciresistens ZS79~T（96%）, and Lysobacter defluii APB-9 ~T（96%）. ~The value for DNA-DNA relatedness between strain 2-5~~T and L. concretionis KC~TC 12205 ~T was 23%. Branched fatty acids iso-C16： 0, iso-C15： 0, iso-C11： 0 3-OH, iso-C17： 1ω9c and iso-C11： 0 were found to be predominant. ~The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Strain 2-5~T had a DNA G＋C content of 63.8 mol%. On the basis of the phenotypic, chemotaxonomic, DNA-DNA hybridization and phylogenetic data, strain 2-5~T represents a novel species of the genus Lysobacter, for which the name Lysobacter hymeniacidonis sp. nov. is proposed. ~The type strain is 2-5~T（=CGMCC 1.12190 ~T = JCM 18137~T）.