Simple Immunoassay for Detection of PCBs in Transformer Oil

A rapid and inexpensive procedure to detect polychlorinated biphenyls (PCBs) in transformer oil is needed to facilitate identification and removal of PCB contaminated transformers. Here we describe a simple two-step liquid−liquid extraction using acidic dimethyl sulfoxide in conjunction with an immu...

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Bibliographic Details
Published in:Environmental science & technology 2005-07, Vol.39 (13), p.5005-5009
Main Authors: Glass, Thomas R, Ohmura, Naoya, Taemi, Yukihiro, Joh, Takashi
Format: Article
Language:English
Subjects:
Applied sciences
Environmental Monitoring - methods
Environmental Pollutants - analysis
Exact sciences and technology
False Negative Reactions
Immunoassay
Immunoassay - methods
Oil
Oils
Other wastes and particular components of wastes
PCB
Pollution
Polychlorinated biphenyls
Polychlorinated Biphenyls - analysis
Power Plants
Sensitivity and Specificity
Transformers
Wastes
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Summary:A rapid and inexpensive procedure to detect polychlorinated biphenyls (PCBs) in transformer oil is needed to facilitate identification and removal of PCB contaminated transformers. Here we describe a simple two-step liquid−liquid extraction using acidic dimethyl sulfoxide in conjunction with an immunoassay for detecting PCBs in transformer oil. The process described is faster and simpler than any previous immunoassay while maintaining comparable detection limit and false negative rate. Cross reactivity data, characterizing the immunoassay response to the four Kanechlor technical mixtures of PCBs in oil, are presented. Forty-five used transformer oil samples were analyzed by gas chromatography−high-resolution mass spectrometry and were also evaluated using the immunoassay protocol developed. Results presented show zero false negatives at a 1.4 ppm nominal cutoff for the transformer oils analyzed.
ISSN:0013-936X
1520-5851
DOI:10.1021/es048012y