Molecular characterization of a new serovar of Salmonella bongori 13,22:z sub(39):- isolated from a lizard

Three Salmonella strains isolated from a lizard (Gallotia simoni) in the "Isla del Hierro" (Canary Islands, Spain) were serotyped as Salmonella bongori serotype 13,22:z sub(39):-, which has not been described in the Kauffmann-White scheme of Salmonella serovars. In order to shed light on t...

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Bibliographic Details
Published in:Research in microbiology 2005-05, Vol.156 (4), p.597-602
Main Authors: Herrera-Leon, Silvia, Saco, Montserrat, Silvestre, Albert Martinez, Silveira, Luis, Echeita, Aurora, Usera, Miguel Angel
Format: Article
Language:English
Subjects:
Gallotia
Gallotia simoni
Lacertilia
Salmonella bongori
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Summary:Three Salmonella strains isolated from a lizard (Gallotia simoni) in the "Isla del Hierro" (Canary Islands, Spain) were serotyped as Salmonella bongori serotype 13,22:z sub(39):-, which has not been described in the Kauffmann-White scheme of Salmonella serovars. In order to shed light on the assignment of those strains to the S. bongori species, several genes were amplified and/or sequenced. The iroB gene has been reported to be present only in S. enterica, while the invA gene has been described as being a helpful tool in distinguishing Salmonella from other bacterial species. Both genes were amplified and, as expected, only invA could be amplified. The fliC gene, encoding the phase 1 flagellin fljB gene, encoding phase 2 flagellin, and the gapA gene, which is believed to present polymorphic alleles among different subspecies, were amplified and sequenced. The sequence obtained from fliC super(z39) matched with the sequences fliC super(z39) obtained from other serovars. The sequence obtained from gapA clustered into the S. bongori group when it was compared to others previously described. We conclude that these three isolates are members of the S. bongori species representing a new serovar that will be described in the next supplement to the Kauffmann-White scheme.
ISSN:0923-2508
DOI:10.1016/j.resmic.2005.01.006