Nuclear heterogeneity in conidial populations of Aspergillus flavus

• Published in: Fungal genetics and biology 2015-11, Vol.84, p.62-72
• Main Authors: Runa, Farhana, Carbone, Ignazio, Bhatnagar, Deepak, Payne, Gary A
• Format: Article
• Language: English
• Subjects: Aspergillus flavus; Aspergillus flavus - genetics; Cell Fusion - methods; Diploidy; Flow Cytometry; Genetic Variation; Genotype; Green Fluorescent Proteins - chemistry; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Luminescent Proteins - chemistry; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Microscopy, Fluorescence; Nucleosomes; Ploidies; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Spores, Fungal - genetics
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1016/j.fgb.2015.09.003

Aspergillus flavus is a major producer of aflatoxin and an opportunistic pathogen for a wide range of hosts. Understanding genotypic and phenotypic variation within strains of A. flavus is important for controlling disease and reducing aflatoxin contamination. A. flavus is multinucleate and predominantly haploid (n) and homokaryotic. Although cryptic heterokaryosis may occur in nature, it is unclear how nuclei in A. flavus influence genetic heterogeneity and if nuclear condition plays a role in fungal ecology. A. flavus mainly reproduces asexually by producing conidia. In order to observe whether conidia are homokaryotic or heterokaryotic, we labeled nuclei of A. flavus using two different nuclear localized fluorescent reporters. The reporter constructs (pYH2A and pCH2B), encode histones HH2A and HH2B fused at the C terminus with either yellow (EYFP) or cyan (ECFP) fluorescent proteins, respectively. The constructs were transformed into the double auxotrophic strain AFC-1 (-pyrG, -argD) to generate a strain containing each reporter construct. By taking advantage of the nutritional requirement for each strain, we were able to generate fusants between FR36 (-argD) expressing yellow fluorescence, and FR46 (-pyr4) expressing cyan fluorescence. Conidia from fusants between FR36 and FR46 showed three types of fluorescence: only EYFP, only ECFP or both EYFP+ECFP. Conidia containing nuclei expressing EYFP+ECFP were separated by Fluorescence-Activated Cell sorting (FACS) and were found to contain both yellow and cyan fluorescent markers in the same nucleus. Further characterization of conidia having only one nucleus but expressing both EYFP+ECFP fluorescence were found to be diploid (2n). Our findings suggest that A. flavus maintains nuclear heterogeneity in conidial populations.