Neurotoxic effect on two fish species and a PC12 cell line of the supernate of Vibrio alginolyticus and Vibrio anguillarum

The biological effects of supernates obtained from different strains of Vibrio alginolyticus and Vibrio anguillarum isolated from diseased fish have been studied by inoculation on two fish species, eel and rainbow trout, and two fish cell lines. These supernates possess neuroexcitatory properties, a...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary microbiology 1998-08, Vol.63 (1), p.61-69
Main Authors: Balebona, M.C, Krovacek, K, Moriñigo, M.A, Mansson, I, Faris, A, Borrego, J.J
Format: Article
Language:English
Subjects:
ACETILCOLINA
ACETYLCHOLINE
Acetylcholine - analysis
ANGUILLA (GENERO)
ANGUILLA (GENRE)
ANGUILLA (GENUS)
Anguilla anguilla
Animals
Cell Survival - drug effects
Culture Media - toxicity
Eel
Eels
Fish
Fish Diseases - microbiology
Fishes - microbiology
Marine
Neurotoxic effects
Neurotoxins - toxicity
ONCORHYNCHUS MYKISS
PC12 cell line
PC12 Cells
Pisces
Rainbow trout
Rats
TOXICIDAD
TOXICITE
TOXICITY
Vibrio - growth & development
Vibrio - isolation & purification
Vibrio alginolyticus
VIBRIO ANGUILLARUM
Vibrio Infections - microbiology
Vibrio Infections - veterinary
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The biological effects of supernates obtained from different strains of Vibrio alginolyticus and Vibrio anguillarum isolated from diseased fish have been studied by inoculation on two fish species, eel and rainbow trout, and two fish cell lines. These supernates possess neuroexcitatory properties, and so, when they are injected into both fish species, they trigger convulsions, wriggling, contortive swimming and respiratory arrest coupled with increased respiratory reflex. Furthermore, after the application of the supernates on cultures of noradrenergic pheochromocytoma PC12 cells, an increase of acetylcholine, released from the cells was obtained. The amount of released acetylcholine depends on the source of assayed supernates and on the dose applied to the cells. On the basis of the results obtained with PC12 cells, we suggest that the supernates from pathogenic Vibrio strains injected into fish may elicit an increased release of acetylcholine in the motor end-plate of some muscles related to locomotion and ventilation of the inoculated fish.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(98)00227-2