THE GREEN FLUORESCENT PROTEIN

In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal f...

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Bibliographic Details
Published in:Annual review of biochemistry 1998-01, Vol.67 (1), p.509-544
Main Author: Tsien, Roger Y
Format: Article
Language:English
Subjects:
Aequorea
Aequorea victoria
Biochemistry
Bioluminescence
Biomarkers
Biosensors
chromophore
Cloning
Genes
Genes, Reporter
Genetic engineering
GFP
green fluorescent protein
Green Fluorescent Proteins
Indicators and Reagents - chemistry
Luminescent Proteins - chemistry
Marine
mutants
Peptides
Photochemicals
Proteins
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Summary:In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.
ISSN:0066-4154
1545-4509
DOI:10.1146/annurev.biochem.67.1.509