Effect of time post mortem on the concentration of endotoxin in rat organs: implications for sudden infant death syndrome (SIDS)

The aim of the study was to test the following hypotheses: (i) that endotoxin injected 40 min prior to death can be detected in rat organs post mortem and (ii) that endotoxin levels do not change with increasing time post mortem. Rats were injected with or without endotoxin in buffered saline, 40 mi...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 1999-08, Vol.25 (1), p.125-130
Main Authors: Sayers, Nicola M., Crawley, Barbara A., Humphries, Kevin, Drucker, David B., Oppenheim, Beryl A., Hunt, Linda P., Morris, James A., Telford, David R.
Format: Article
Language:English
Subjects:
Endotoxin
Postmortem
Rat
Sudden infant death syndrome
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Summary:The aim of the study was to test the following hypotheses: (i) that endotoxin injected 40 min prior to death can be detected in rat organs post mortem and (ii) that endotoxin levels do not change with increasing time post mortem. Rats were injected with or without endotoxin in buffered saline, 40 min prior to being killed. Endotoxin levels in rat organs were assessed using a Limulus amoebocyte assay. The effect of storage time post mortem was assessed by following various storage regimes at 25°C and 8°C. Significant differences ( P=
ISSN:0928-8244
1574-695X
DOI:10.1016/S0928-8244(99)00080-2