Identification of a soluble leptin receptor in crucian carp with different binding affinity to leptin-a and leptin-b

Soluble leptin receptor (sLepR) is the main leptin-binding protein in plasma and contributes to activation of circulating leptin. In this study, we identified a sLepR in plasma of crucian carp (Carassius carassius) using a pull-down assay, and the interaction of sLepR with its ligand is confirmed by...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2016-01, Vol.191, p.108-111
Main Authors: Xie, Feifei, Li, Xin, Huang, Saifan, Li, Jiyuan, Guo, Xiaopin, Cao, Yibin
Format: Article
Language:English
Subjects:
Animals
Carps - blood
Carps - metabolism
Crucian carp
Fish Proteins - chemistry
Fish Proteins - metabolism
Leptin - metabolism
Leptin-a
Leptin-b
Protein Binding
Receptors, Leptin - chemistry
Receptors, Leptin - metabolism
Solubility
Soluble leptin receptor
Substrate Specificity
Teleost
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Summary:Soluble leptin receptor (sLepR) is the main leptin-binding protein in plasma and contributes to activation of circulating leptin. In this study, we identified a sLepR in plasma of crucian carp (Carassius carassius) using a pull-down assay, and the interaction of sLepR with its ligand is confirmed by a cross-linking study. In addition, we found that leptin-a has higher affinity than leptin-b for sLepR. According to our knowledge, this is the first experimental report about the main ligand of sLepR in teleost.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2015.10.003