Defluvibacter lusatiae gen. nov., sp. nov., a New Chlorophenol-degrading Member of the α-2 Subgroup of Proteobacteria

• Published in: Systematic and applied microbiology 1999-05, Vol.22 (2), p.197-204
• Main Authors: Fritsche, Kathrin, Auling, Georg, Andreesen, Jan R., Lechner, Ute
• Format: Article
• Language: English
• Subjects: 2,4-dichlorophenol; 4-chloro-2-methylphenol; 4-chlorophenol; Bacteriology; biodegradation; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; chemotaxonomy; chlorophenols; Defluvibacter lusatiae; descriptions; Fundamental and applied biological sciences. Psychology; Gram-negative bacteria; Isolation and description; Microbiology; Mission oriented research; new genus; new species; organochlorine compounds; phenol; phenols; phylogeny; Systematics; α-2 subgroup of Proteobacteria
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/S0723-2020(99)80066-6

The two Gram-negative bacterial strains S1 and S4 were isolated from activated sludge of an industrial waste water treatment plant and exhibited a stable capability to degrade 2,4-dichlorophenol, 4-chloro-2-methylphenol, 4-chlorophenol and phenol. The cells were short rods with a polar flagellum, being mesophilic, strictly aerobic, oxidase-positive, and chemoorganotrophic. They utilized a range of amino acids, but only a restricted number of carbohydrates. Reassociation experiments with DNA from strains S1 and S4 revealed high interstrain similarity, indicating, that both strains belong to the same species. The phylogenetic position was determined by comparison of the almost complete 16S rDNA sequence of strain S1 with sequences of related bacteria. Strain S1 clustered with members of the α-2 subgroup of the Proteobacteria by forming a separate lineage within the radiation of Mesorhizobium, Phyllobacterium and Sinorhizobium. Both strains can be differentiated from members of related taxa by a set of physiological and chemotaxonomic properties including the ability to grow with norvaline, L-tryptophan, putrescine, glutarate and malonate, and by the presence of spermidine as major polyamine and of 12:0 3OH as fatty acid. Strain S1 is described as type strain of a new species and assigned to a new genus with the proposed name Defluvibacter lusatiae.