Importance of srtA and srtB for Growth of Bacillus anthracis in Macrophages

We examined the effect of mutation of two sortase genes of Bacillus anthracis, srtA and srtB, on the ability of the bacterium to grow in J774A.1 cells, a mouse macrophage-like cell line. While disruption of either srtA or srtB had no effect on the ability of the bacteria to grow in rich culture medi...

Full description

Saved in:
Bibliographic Details
Published in:Infection and Immunity 2005-08, Vol.73 (8), p.5222-5228
Main Authors: Zink, Steven D, Burns, Drusilla L
Format: Article
Language:English
Subjects:
Aminoacyltransferases - genetics
Aminoacyltransferases - metabolism
Animals
Anthrax - enzymology
Anthrax - microbiology
Bacillus anthracis
Bacillus anthracis - enzymology
Bacillus anthracis - genetics
Bacillus anthracis - growth & development
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Cysteine Endopeptidases
Fundamental and applied biological sciences. Psychology
Macrophages - microbiology
Mice
Microbiology
Sequence Analysis, DNA
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We examined the effect of mutation of two sortase genes of Bacillus anthracis, srtA and srtB, on the ability of the bacterium to grow in J774A.1 cells, a mouse macrophage-like cell line. While disruption of either srtA or srtB had no effect on the ability of the bacteria to grow in rich culture media, mutations in each of these genes dramatically attenuated growth of the bacterium in J774A.1 cells. Complementation of the mutation restored the ability of bacteria to grow in the cells. Since the initial events in inhalation anthrax are believed to be uptake of B. anthracis spores by alveolar macrophages followed by germination of the spores and growth of the bacteria within the macrophages, these results suggest that two sortases of B. anthracis may be critical in the early stages of inhalation anthrax.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.8.5222-5228.2005