Location of prunus necrotic ringspot ilarvirus within pollen grains of infected nectarine trees: evidence from RT-PCR, dot-blot and in situ hybridisation

Prunus necrotic ringspot Ilarvirus (PNRSV) is a pollen and seed-borne ilarvirus affecting most Prunus spp. The location of the virus in infected nectarine pollen grains was investigated by molecular hybridisation, RT-PCR and in situ hybridisation. The first two approaches revealed an internal locati...

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Bibliographic Details
Published in:European journal of plant pathology 1999-09, Vol.105 (6), p.623-627
Main Authors: Aparicio, F. (Centro de Edafologia y Biologia Aplicada de Segura CSIC, Murcia (Spain). Dept. de Mejora y Patologia Vegetal), Sanchez-Pina, M.A, Sanchez-Navarro, J.A, Pallas, V
Format: Article
Language:English
Subjects:
ANTERA
ANTHERE
ANTHERS
Biological and medical sciences
DISEASE TRANSMISSION
DNA HYBRIDIZATION
Epidemiology
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
HIBRIDACION DE ADN
HYBRIDATION D'ADN
ILARVIRUS
ILARVIRUSES
Microbiology
PCR
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
POLEN
POLLEN
pollen-borne diseases
PRUNUS
Prunus necrotic ringspot virus
TRANSMISION DE ENFERMEDADES
TRANSMISSION DES MALADIES
Virology
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Summary:Prunus necrotic ringspot Ilarvirus (PNRSV) is a pollen and seed-borne ilarvirus affecting most Prunus spp. The location of the virus in infected nectarine pollen grains was investigated by molecular hybridisation, RT-PCR and in situ hybridisation. The first two approaches revealed an internal location of the virus. In situ hybridisation demonstrated the virus in the bicellular pollen grain, where it was present in the cytoplasm of the vegetative cell but not in the generative cell. This result seems to indicate that the sperm cells, formed by the mitosis of the generative cell, are not involved in virus transmission to seed. Other possible mechanisms are discussed.[PUBLICATION ABSTRACT]
ISSN:0929-1873
1573-8469
DOI:10.1023/A:1008783705183