Use of Rotavirus Virus-Like Particles as Surrogates To Evaluate Virus Persistence in Shellfish

Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log₁₀ of VLPs wa...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2005-10, Vol.71 (10), p.6049-6053
Main Authors: Loisy, Fabienne, Atmar, Robert L, Le Saux, Jean-Claude, Cohen, Jean, Caprais, Marie-Paule, Pommepuy, Monique, Le Guyader, Françoise S
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - metabolism
Atoms & subatomic particles
bacteriophages
Biological and medical sciences
Brackish
Capsid Proteins - metabolism
Crassostrea gigas
depuration
Environmental Monitoring - methods
Fundamental and applied biological sciences. Psychology
indicator species
Levivirus - growth & development
Levivirus - isolation & purification
Marine
microbial contamination
Microbiology
Ostreidae - physiology
Ostreidae - virology
oysters
Pancreas - virology
pathogen survival
Phage MS2
Public Health Microbiology
Regression analysis
Rotavirus
Rotavirus - growth & development
Rotavirus - isolation & purification
Shellfish
Shellfish - virology
Virion - growth & development
Virion - isolation & purification
Virion - metabolism
virus-like particles
Viruses
water pollution
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Summary:Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log₁₀ of VLPs was depurated after 1 week in warm seawater (22°C). Phage MS2 was depurated more rapidly (about 2 log₁₀ in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10¹⁰ and 10⁹ particles per g of pancreatic tissue) and for 37 days for lower contamination levels (10⁵ particles per g of pancreatic tissue). These data suggest that viral particles may persist in shellfish tissues for several weeks.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.10.6049-6053.2005