Expression of TIMP-1 and -2 in different growth patterns of adenoid cystic carcinoma

Tissue inhibitors of metalloproteinases (TIMPs) are special inhibitors of matrix metalloproteinases. To evaluate their roles in adenoid cystic carcinoma (ACC), we compared TIMP-1 and -2 mRNA and protein expression in different histological pattern of ACC. We obtained carcinoma cells from each of cri...

Full description

Saved in:
Bibliographic Details
Published in:Oral oncology 2005-09, Vol.41 (8), p.821-827
Main Authors: Wang, Yan, Irié, Tarou, Aida, Tadateru, Tachikawa, Tetsuhiko
Format: Article
Language:English
Subjects:
Adenoid cystic carcinoma
Aged
Biological and medical sciences
Carcinoma, Adenoid Cystic - metabolism
Carcinoma, Adenoid Cystic - pathology
Female
Growth pattern
Humans
Immunohistochemistry
Laser microdissection
Male
Medical sciences
Middle Aged
Mouth Neoplasms - metabolism
Mouth Neoplasms - pathology
Neoplasm Proteins - metabolism
Otorhinolaryngology. Stomatology
Real-time reverse transcription-polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Neoplasm - metabolism
Tissue Inhibitor of Metalloproteinase-1 - metabolism
Tissue Inhibitor of Metalloproteinase-2 - metabolism
Tissue inhibitor of metalloproteinases
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tissue inhibitors of metalloproteinases (TIMPs) are special inhibitors of matrix metalloproteinases. To evaluate their roles in adenoid cystic carcinoma (ACC), we compared TIMP-1 and -2 mRNA and protein expression in different histological pattern of ACC. We obtained carcinoma cells from each of cribriform and tubular pattern of ACCs using by laser microdissection (LM), to determine the mRNAs expression of TIMP-1 and -2 using by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR), and to confirm expression of them by immunohistochemical staining. Our results showed that mRNA expression of TIMP-1 tended to be decreased in cribriform pattern compared with tubular pattern in four cases, and TIMP-1 significantly decreased in three cases. TIMP-2 also significantly decreased in cribriform than in tubular pattern in three of four cases. Protein expression of TIMP-1 and -2 decreased in the cribriform pattern compared to tubular pattern. These results indicate that there is close relationship between TIMPs and growth patterns of ACC, and TIMP-1 and -2 may play important roles in morphogenesis and biological character of adenoid cystic carcinoma.
ISSN:1368-8375
1879-0593
DOI:10.1016/j.oraloncology.2005.04.009