Species diversity in the Antrodia crassa group (Polyporales, Basidiomycota)

• Published in: Fungal biology 2015-12, Vol.119 (12), p.1291-1310
• Main Authors: Spirin, Viacheslav, Runnel, Kadri, Vlasák, Josef, Miettinen, Otto, Põldmaa, Kadri
• Format: Article
• Language: English
• Subjects: Antrodia - classification; Antrodia - genetics; Antrodia - isolation & purification; Biodiversity; Host specificity; Internal transcribed spacer; Molecular Sequence Data; Phylogeny; Polypores; Taxonomy; Translation elongation factor; Trees - microbiology; Wood - microbiology; Wood-inhabiting fungi
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2015.09.008

Antrodia is a polyphyletic genus, comprising brown-rot polypores with annual or short-lived perennial resupinate, dimitic basidiocarps. Here we focus on species that are closely related to Antrodia crassa, and investigate their phylogeny and species delimitation using geographic, ecological, morphological and molecular data (ITS and LSU rDNA, tef1). Phylogenetic analyses distinguished four clades within the monophyletic group of eleven conifer-inhabiting species (five described herein): (1)A. crassa s. str. (boreal Eurasia), Antrodia cincta sp. nova (North America) and Antrodia cretacea sp. nova (holarctic), all three being characterized by inamyloid skeletal hyphae that dissolve quickly in KOH solution; (2) Antrodia ignobilis sp. nova, Antrodia sitchensis and Antrodia sordida from North America, and Antrodia piceata sp. nova (previously considered conspecific with A. sitchensis) from Eurasia, possessing amyloid skeletal hyphae; (3) Antrodia ladiana sp. nova from the southern part of the USA, Antrodia pinea from East Asia, and Antrodia ferox – so far known from subtropical North America, but here reported also from Eurasia. These three species have inamyloid hyphae and narrow basidiospores; (4) the North American Antrodia pini-cubensis, sharing similar morphological characters with A. pinea, forming a separate clade. The habitat data indicate that several species are threatened by intensive forestry. •We revised the taxonomy of the Antrodia crassa group (brown-rot polypores).•We described five new species based on molecular, ecological, and geographic characters.•Several species in A. crassa group appear rare and threatened by forestry.•Taxonomic revision in published lists of threatened and indicator species is needed.