Sequence, assembly and analysis of pX01 and pX02

Bacillus anthracis plasmids pX01 and pX02, harboured by the Sterne and Pasteur strains, respectively, have been sequenced by random ‘shotgun’ cloning and high throughout sequence analysis. These sequences have been assembled (Sequencher) to generate a circulate pX01 plasmid containing 181 656 bp and...

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Bibliographic Details
Published in:Journal of applied microbiology 1999-08, Vol.87 (2), p.261-262
Main Authors: Okinaka, R., Cloud, K., Hampton, O., Hoffmaster, A., Hill, K., Keim, P., Koehler, T., Lamke, G., Kumano, S., Manter, D., Martinez, Y., Ricke, D., Svensson, R., Jackson, P.
Format: Article
Language:English
Subjects:
Bacillus anthracis
Bacillus anthracis - genetics
Bacteriology
Biological and medical sciences
cya gene
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
lef gene
Microbiology
Open Reading Frames - genetics
pag gene
plasmid pX01
plasmid pX02
Plasmids - genetics
Sequence Analysis
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Summary:Bacillus anthracis plasmids pX01 and pX02, harboured by the Sterne and Pasteur strains, respectively, have been sequenced by random ‘shotgun’ cloning and high throughout sequence analysis. These sequences have been assembled (Sequencher) to generate a circulate pX01 plasmid containing 181 656 bp and a single linear (gapped) pX02 contig containing at least 93·479 bp. Initial annotation suggests that the two plasmids combined contain at least 200 potential open reading frames (ORFs) with
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1999.00883.x