Production and characterization of Amanitin toxins from a pure culture of Amanita exitialis

Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear r...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-11, Vol.252 (2), p.223-228
Main Authors: Zhang, Ping, Chen, ZuoHong, Hu, JinSong, Wei, BaoYang, Zhang, ZhiGuang, Hu, WenQi
Format: Article
Language:English
Subjects:
Amanita
Amanita - cytology
Amanita - genetics
Amanita - growth & development
Amanita - metabolism
Amanita exitialis
Amanitin
Amanitins - analysis
Amanitins - biosynthesis
Amanitins - chemistry
Amanitins - isolation & purification
Amatoxins
Biological and medical sciences
Chromatography, High Pressure Liquid
Culture
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Intergenic - chemistry
DNA, Intergenic - genetics
Dry matter
Fruit bodies
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
High performance liquid chromatography
HPLC
ITS
Liquid chromatography
Mass Spectrometry
Mass spectroscopy
Microbiology
Molecular Sequence Data
Mushrooms
Mycology
Mycotoxins - chemistry
Mycotoxins - isolation & purification
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Photomicrography
Phylogeny
Pure culture
Ribosomal DNA
Sequence Analysis, DNA
Species
Toxins
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Summary:Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the pure culture and of a typical fruiting body of the species were sequenced and compared. Further, amatoxins produced by pure cultures were analyzed and characterized by high-performance liquid chromatography and mass spectrometry analysis. The results showed that the pure cultures produced 728.3 ± 43.8 μg g −1 (dry matter) of α-Amanitin and 60.0 ± 20.7 μg g −1 (dry matter) of β-Amanitin, respectively, a yield which is about 10% of that produced by fruiting bodies.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.08.049