Sequence analysis of a new open reading frame located in the pathogenicity locus of Clostridium difficile strain 8864

Strain 8864 is a natural isolate of Clostridium difficile that is toxin B-positive and toxin A-negative. Recent work showed that there is a genetic rearrangement occurring at the pathogenicity locus (PaLoc) of the bacteria. Our investigation in the PaLoc region revealed an open reading frame ( tcdF)...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-11, Vol.180 (2), p.241-248
Main Authors: Song, Keang Peng, Ow, Sui Eng, Chang, Siao Yun, Bai, Xin Lai
Format: Article
Language:English
Subjects:
Clostridium difficile
Recombinant DNA
Sequence analysis
tcdF gene
Toxinogenic Clostridium difficile
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Summary:Strain 8864 is a natural isolate of Clostridium difficile that is toxin B-positive and toxin A-negative. Recent work showed that there is a genetic rearrangement occurring at the pathogenicity locus (PaLoc) of the bacteria. Our investigation in the PaLoc region revealed an open reading frame ( tcdF) of 543 bp DNA not reported before. This tcdF could encode a putative polypeptide of 22 kDa. Although no peptide homology was found with other known proteins, we postulate that it could be a novel protein because not only highly consensus ribosome-binding and promoter sequences were found upstream of tcdF, transcript was also identified at the region occupied by tcdF.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(99)00491-7