Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus

Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of cry...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-11, Vol.252 (1), p.51-56
Main Authors: El-Bendary, Magda, Priest, Fergus G., Charles, Jean-François, Mitchell, Wilfrid J.
Format: Article
Language:English
Subjects:
Animals
Anopheles - microbiology
Asporogenic mutants
Bacillus - genetics
Bacillus - metabolism
Bacillus - pathogenicity
Bacillus - physiology
Bacillus sphaericus
Bacillus subtilis
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Conjugation
Crystal proteins
Crystals
E coli
Electron microscopy
Escherichia coli
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Gene expression
Gene Expression Regulation, Bacterial
Genes
Growth, nutrition, cell differenciation
Insecticidal toxin
Larva - microbiology
Larvae
Microbiology
Mutation
Protein biosynthesis
Protein synthesis
Proteins
Sigma Factor - genetics
Sigma Factor - metabolism
Sodium lauryl sulfate
Spores, Bacterial - genetics
Spores, Bacterial - physiology
Sporulation
Suicide
Toxicity
Transcription Factors - metabolism
Transcription, Genetic
Western blotting
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Summary:Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS–PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.08.027