Loading…
Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus
Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of cry...
Saved in:
| Published in: | FEMS microbiology letters 2005-11, Vol.252 (1), p.51-56 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c5371-e623cc7a3748ace0ca5202b3e3b968cd3e68093700ae17e242e5187a5e7a88213 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c5371-e623cc7a3748ace0ca5202b3e3b968cd3e68093700ae17e242e5187a5e7a88213 |
| container_end_page | 56 |
| container_issue | 1 |
| container_start_page | 51 |
| container_title | FEMS microbiology letters |
| container_volume | 252 |
| creator | El-Bendary, Magda Priest, Fergus G. Charles, Jean-François Mitchell, Wilfrid J. |
| description | Insertional mutations in the
spo0A and
spoIIAC genes of
Bacillus sphaericus 2362 were prepared by conjugation with
Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their
Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS–PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes. |
| doi_str_mv | 10.1016/j.femsle.2005.08.027 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17404782</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1016/j.femsle.2005.08.027</oup_id><els_id>S0378109705005781</els_id><sourcerecordid>17404782</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5371-e623cc7a3748ace0ca5202b3e3b968cd3e68093700ae17e242e5187a5e7a88213</originalsourceid><addsrcrecordid>eNqNkdGL1DAQxoso3nr6H4gUxHtrb5K0SfoinMudCiu-6HPIprNelmxbM63a_96ULhz4IAeBkOH3fTOTL8teMygZMHl9LA94ooAlB6hL0CVw9STbsFpVhWykfpptQChdMGjURfaC6AgAFQf5PLtgkslacLbJ9ts402hDPsR-RN_lNHfjPZKnPJ0WB-xa7Ma873K0Mcw5DX2cgh19qvzADnP8M0QkWt5J_sE6H8JEibu3GL2b6GX27GAD4avzfZl9v7v9tv1U7L5-_Ly92RWuFooVKLlwTlmhKm0dgrM1B74XKPZpG9cKlBoaoQAsMoW84lgzrWyNymrNmbjMrlbftMrPCWk0J08OQ7Ad9hMZpiqolOYJfPsPeOyn2KXZDBcg67oB2SSqWikXe6KIBzNEf7JxNgzMkoA5mjUBsyRgQJuUQJK9OZtP-xO2D6Lzlyfg3Rmw5Gw4RNs5Tw-cYlpWYjFSK_fbB5wf1dzcfdnVS4frVdlPw2OHfr8qMOXzy2M05Dx2Dlsf0Y2m7f3_Df4CChjHQA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2306559069</pqid></control><display><type>article</type><title>Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus</title><source>Oxford Journals Online</source><creator>El-Bendary, Magda ; Priest, Fergus G. ; Charles, Jean-François ; Mitchell, Wilfrid J.</creator><creatorcontrib>El-Bendary, Magda ; Priest, Fergus G. ; Charles, Jean-François ; Mitchell, Wilfrid J.</creatorcontrib><description>Insertional mutations in the
spo0A and
spoIIAC genes of
Bacillus sphaericus 2362 were prepared by conjugation with
Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their
Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS–PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/j.femsle.2005.08.027</identifier><identifier>PMID: 16165321</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Animals ; Anopheles - microbiology ; Asporogenic mutants ; Bacillus - genetics ; Bacillus - metabolism ; Bacillus - pathogenicity ; Bacillus - physiology ; Bacillus sphaericus ; Bacillus subtilis ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Conjugation ; Crystal proteins ; Crystals ; E coli ; Electron microscopy ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Gel electrophoresis ; Gene expression ; Gene Expression Regulation, Bacterial ; Genes ; Growth, nutrition, cell differenciation ; Insecticidal toxin ; Larva - microbiology ; Larvae ; Microbiology ; Mutation ; Protein biosynthesis ; Protein synthesis ; Proteins ; Sigma Factor - genetics ; Sigma Factor - metabolism ; Sodium lauryl sulfate ; Spores, Bacterial - genetics ; Spores, Bacterial - physiology ; Sporulation ; Suicide ; Toxicity ; Transcription Factors - metabolism ; Transcription, Genetic ; Western blotting</subject><ispartof>FEMS microbiology letters, 2005-11, Vol.252 (1), p.51-56</ispartof><rights>2005 Federation of European Microbiological Societies</rights><rights>2005 Federation of European Microbiological Societies 2005</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5371-e623cc7a3748ace0ca5202b3e3b968cd3e68093700ae17e242e5187a5e7a88213</citedby><cites>FETCH-LOGICAL-c5371-e623cc7a3748ace0ca5202b3e3b968cd3e68093700ae17e242e5187a5e7a88213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17186437$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16165321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Bendary, Magda</creatorcontrib><creatorcontrib>Priest, Fergus G.</creatorcontrib><creatorcontrib>Charles, Jean-François</creatorcontrib><creatorcontrib>Mitchell, Wilfrid J.</creatorcontrib><title>Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Insertional mutations in the
spo0A and
spoIIAC genes of
Bacillus sphaericus 2362 were prepared by conjugation with
Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their
Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS–PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes.</description><subject>Animals</subject><subject>Anopheles - microbiology</subject><subject>Asporogenic mutants</subject><subject>Bacillus - genetics</subject><subject>Bacillus - metabolism</subject><subject>Bacillus - pathogenicity</subject><subject>Bacillus - physiology</subject><subject>Bacillus sphaericus</subject><subject>Bacillus subtilis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Conjugation</subject><subject>Crystal proteins</subject><subject>Crystals</subject><subject>E coli</subject><subject>Electron microscopy</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gel electrophoresis</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Growth, nutrition, cell differenciation</subject><subject>Insecticidal toxin</subject><subject>Larva - microbiology</subject><subject>Larvae</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Sigma Factor - genetics</subject><subject>Sigma Factor - metabolism</subject><subject>Sodium lauryl sulfate</subject><subject>Spores, Bacterial - genetics</subject><subject>Spores, Bacterial - physiology</subject><subject>Sporulation</subject><subject>Suicide</subject><subject>Toxicity</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Western blotting</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkdGL1DAQxoso3nr6H4gUxHtrb5K0SfoinMudCiu-6HPIprNelmxbM63a_96ULhz4IAeBkOH3fTOTL8teMygZMHl9LA94ooAlB6hL0CVw9STbsFpVhWykfpptQChdMGjURfaC6AgAFQf5PLtgkslacLbJ9ts402hDPsR-RN_lNHfjPZKnPJ0WB-xa7Ma873K0Mcw5DX2cgh19qvzADnP8M0QkWt5J_sE6H8JEibu3GL2b6GX27GAD4avzfZl9v7v9tv1U7L5-_Ly92RWuFooVKLlwTlmhKm0dgrM1B74XKPZpG9cKlBoaoQAsMoW84lgzrWyNymrNmbjMrlbftMrPCWk0J08OQ7Ad9hMZpiqolOYJfPsPeOyn2KXZDBcg67oB2SSqWikXe6KIBzNEf7JxNgzMkoA5mjUBsyRgQJuUQJK9OZtP-xO2D6Lzlyfg3Rmw5Gw4RNs5Tw-cYlpWYjFSK_fbB5wf1dzcfdnVS4frVdlPw2OHfr8qMOXzy2M05Dx2Dlsf0Y2m7f3_Df4CChjHQA</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>El-Bendary, Magda</creator><creator>Priest, Fergus G.</creator><creator>Charles, Jean-François</creator><creator>Mitchell, Wilfrid J.</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>200511</creationdate><title>Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus</title><author>El-Bendary, Magda ; Priest, Fergus G. ; Charles, Jean-François ; Mitchell, Wilfrid J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5371-e623cc7a3748ace0ca5202b3e3b968cd3e68093700ae17e242e5187a5e7a88213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Anopheles - microbiology</topic><topic>Asporogenic mutants</topic><topic>Bacillus - genetics</topic><topic>Bacillus - metabolism</topic><topic>Bacillus - pathogenicity</topic><topic>Bacillus - physiology</topic><topic>Bacillus sphaericus</topic><topic>Bacillus subtilis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Conjugation</topic><topic>Crystal proteins</topic><topic>Crystals</topic><topic>E coli</topic><topic>Electron microscopy</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gel electrophoresis</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Growth, nutrition, cell differenciation</topic><topic>Insecticidal toxin</topic><topic>Larva - microbiology</topic><topic>Larvae</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>Protein biosynthesis</topic><topic>Protein synthesis</topic><topic>Proteins</topic><topic>Sigma Factor - genetics</topic><topic>Sigma Factor - metabolism</topic><topic>Sodium lauryl sulfate</topic><topic>Spores, Bacterial - genetics</topic><topic>Spores, Bacterial - physiology</topic><topic>Sporulation</topic><topic>Suicide</topic><topic>Toxicity</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Bendary, Magda</creatorcontrib><creatorcontrib>Priest, Fergus G.</creatorcontrib><creatorcontrib>Charles, Jean-François</creatorcontrib><creatorcontrib>Mitchell, Wilfrid J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Bendary, Magda</au><au>Priest, Fergus G.</au><au>Charles, Jean-François</au><au>Mitchell, Wilfrid J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2005-11</date><risdate>2005</risdate><volume>252</volume><issue>1</issue><spage>51</spage><epage>56</epage><pages>51-56</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Insertional mutations in the
spo0A and
spoIIAC genes of
Bacillus sphaericus 2362 were prepared by conjugation with
Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their
Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS–PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>16165321</pmid><doi>10.1016/j.femsle.2005.08.027</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2005-11, Vol.252 (1), p.51-56 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17404782 |
| source | Oxford Journals Online |
| subjects | Animals Anopheles - microbiology Asporogenic mutants Bacillus - genetics Bacillus - metabolism Bacillus - pathogenicity Bacillus - physiology Bacillus sphaericus Bacillus subtilis Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Conjugation Crystal proteins Crystals E coli Electron microscopy Escherichia coli Fundamental and applied biological sciences. Psychology Gel electrophoresis Gene expression Gene Expression Regulation, Bacterial Genes Growth, nutrition, cell differenciation Insecticidal toxin Larva - microbiology Larvae Microbiology Mutation Protein biosynthesis Protein synthesis Proteins Sigma Factor - genetics Sigma Factor - metabolism Sodium lauryl sulfate Spores, Bacterial - genetics Spores, Bacterial - physiology Sporulation Suicide Toxicity Transcription Factors - metabolism Transcription, Genetic Western blotting |
| title | Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T01%3A36%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Crystal%20protein%20synthesis%20is%20dependent%20on%20early%20sporulation%20gene%20expression%20in%20Bacillus%20sphaericus&rft.jtitle=FEMS%20microbiology%20letters&rft.au=El-Bendary,%20Magda&rft.date=2005-11&rft.volume=252&rft.issue=1&rft.spage=51&rft.epage=56&rft.pages=51-56&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1016/j.femsle.2005.08.027&rft_dat=%3Cproquest_cross%3E17404782%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5371-e623cc7a3748ace0ca5202b3e3b968cd3e68093700ae17e242e5187a5e7a88213%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2306559069&rft_id=info:pmid/16165321&rft_oup_id=10.1016/j.femsle.2005.08.027&rfr_iscdi=true |