Identification of mRNAs localizing in the postsynaptic region

Local protein synthesis using mRNAs readily distributed in the dendrites is believed to play an important role in maintaining the already expressed synaptic plasticity. To find proteins translated in the postsynaptic region, such as neuronal dendrites, we tried to identify the mRNAs associated with...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 1999-10, Vol.72 (2), p.147-157
Main Authors: Tian, Qing Bao, Nakayama, Kohzo, Okano, Akira, Suzuki, Tatsuo
Format: Article
Language:English
Subjects:
Anatomy
Animals
Biological and medical sciences
Central nervous system
Dendrite
Dendrites - metabolism
DNA, Complementary - genetics
Excitatory Amino Acid Agonists - pharmacology
Excitatory Amino Acid Agonists - toxicity
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Kainic Acid - pharmacology
Kainic Acid - toxicity
Local protein synthesis
Male
mRNA
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - genetics
Postsynaptic density
Prosencephalon - chemistry
Prosencephalon - drug effects
Protein Biosynthesis
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Synaptic plasticity
Vertebrates: nervous system and sense organs
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Summary:Local protein synthesis using mRNAs readily distributed in the dendrites is believed to play an important role in maintaining the already expressed synaptic plasticity. To find proteins translated in the postsynaptic region, such as neuronal dendrites, we tried to identify the mRNAs associated with the postsynaptic density (PSD) fraction prepared from a rat's forebrain. The PSD-associated mRNAs were amplified by reverse transcriptase-based polymerase chain reaction (RT-PCR), separated by polyacrylamide gel electrophoresis, and sequenced. The database search revealed, among 130 mRNAs sequenced, 17 known and 108 unknown sequences, while five mRNAs were too short for the search. Of the mRNAs with unknown sequences, we selected 33 genes with a length longer than 150 bases, performed in situ hybridization, and found that at least 12 mRNA types were localized in the dendrites. These results suggest that a large number of mRNAs localize around the postsynaptic area of the neuronal cells in the central nervous system. In addition, our method proved efficient in identifying collectively the mRNAs localizing in the dendrites.
ISSN:0169-328X
1872-6941
DOI:10.1016/S0169-328X(99)00214-4