Increased Expression of Rat Synuclein in the Substantia Nigra Pars Compacta Identified by mRNA Differential Display in a Model of Developmental Target Injury

• Published in: Journal of neurochemistry 1999-12, Vol.73 (6), p.2586-2599
• Main Authors: Kholodilov, Nikolai G., Neystat, Michael, Tinmarla, F. Oo, Steven, E. Lo, Larsen, Kristin E., Sulzer, David, Burke, Robert E.
• Format: Article
• Language: English
• Subjects: alpha-Synuclein; Amino Acid Sequence; Animals; Apoptosis; Apoptosis - drug effects; Base Sequence; Biological and medical sciences; Blotting, Northern; Blotting, Western; Cells, Cultured; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Dopamine - metabolism; Dopaminergic neurons; Gene Expression Profiling; Gene Expression Regulation, Developmental; Genes; In Situ Hybridization; Medical sciences; Molecular Sequence Data; Nerve Degeneration - chemically induced; Nerve Degeneration - genetics; Nerve Degeneration - metabolism; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; Neurology; Neurons - drug effects; Neurons - metabolism; Neurotoxins - toxicity; Parkinson Disease - metabolism; Parkinson's disease; Programmed cell death; Quinolinic Acid - toxicity; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA Splicing; RNA, Antisense - pharmacology; RNA, Messenger - biosynthesis; Substantia nigra; Substantia Nigra - drug effects; Substantia Nigra - growth & development; Substantia Nigra - metabolism; Substantia Nigra - pathology; Subtraction Technique; Synuclein; Synucleins
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1046/j.1471-4159.1999.0732586.x

: Human α‐synuclein was identified on the basis of proteolytic fragments derived from senile plaques of Alzheimer's disease, and it is the locus of mutations in some familial forms of Parkinson's disease. Its normal function and whether it may play a direct role in neural degeneration remain unknown. To explore cellular responses to neural degeneration in the dopamine neurons of the substantia nigra, we have developed a rodent model of apoptotic death induced by developmental injury to their target, the striatum. We find by mRNA differential display that synuclein is up‐regulated in this model, and thus it provides an opportunity to examine directly whether synuclein plays a role in the death of these neurons or, alternatively, in compensatory responses. Up‐regulation of mRNA is associated with an increase in the number of neuronal profiles immunostained for synuclein protein. At a cellular level, synuclein is almost exclusively expressed in normal neurons, rather than apoptotic profiles. Synuclein is up‐regulated throughout normal postnatal development of substantia nigra neurons, but it is not further up‐regulated during periods of natural cell death. We conclude that up‐regulation of synuclein in the target injury model is unlikely to mediate apoptotic death and propose that it may be due to a compensatory response in neurons destined to survive.