Identification of new CNS-resident macrophage subpopulation molecular markers for the discrimination with murine systemic macrophages

• Published in: Journal of neuroimmunology 2005-12, Vol.169 (1), p.39-49
• Main Authors: Donnou, Sabrina, Fisson, Sylvain, Mahe, Dominique, Montoni, Alicia, Couez, Dominique
• Format: Article
• Language: English
• Subjects: Adult microglia; Animals; Antigens, CD - classification; Antigens, CD - metabolism; Biomarkers - metabolism; Blotting, Northern - methods; Cells, Cultured; Central Nervous System - cytology; cRDA; Discriminant mRNA; DNA, Complementary - metabolism; EOC; Flow Cytometry - methods; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Glial Fibrillary Acidic Protein - metabolism; Interferon-alpha - pharmacology; Interferon-gamma - pharmacology; Interleukin-10 - pharmacology; Macrophage Activation - physiology; Macrophages - metabolism; Mice; Mice, Inbred C3H; Microglia - metabolism; Monocytes; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - metabolism; Splenic and peritoneal macrophages
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2005.07.016

A controversial issue in neurobiology concerns the respective functions of central nervous system (CNS)-resident macrophages and systemic infiltrating macrophages morphologically and phenotypically similar during most of CNS injury processes. In a previous work, we isolated sixteen mRNAs differentially expressed between two microglial EOC clones. By studying their pattern of expression, we found that three of them were not expressed in peripheral macrophages, even after stimulation with IFNγ, TNFα or IL10. These three molecules are physiologically expressed by murine adult microglia and could be used to evaluate in vivo their discriminative potential toward CNS-infiltrating macrophages during inflammatory events.