Characterization of a Multicopper Oxidase Gene from Staphylococcus aureus

A multicopper oxidase gene from Staphylococcus aureus was cloned and overexpressed. Purified recombinant multicopper oxidase oxidized the substrate 3,3'-dimethoxybenzidine in the presence of copper. Disruption of mco showed copper sensitivity and H₂O₂ resistance, suggesting roles for mco in cop...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2005-09, Vol.71 (9), p.5650-5653
Main Authors: Sitthisak, S, Howieson, K, Amezola, C, Jayaswal, R. K
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Biological and medical sciences
Cloning, Molecular
Copper
Copper - metabolism
Copper - pharmacology
Dianisidine - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genes
Genetics
Genetics and Molecular Biology
Heat-Shock Response
Hydrogen Peroxide - pharmacology
Microbiology
Molecular Sequence Data
Oxidation-Reduction
Oxidative Stress
Oxidoreductases - genetics
Oxidoreductases - metabolism
Sequence Analysis, DNA
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - enzymology
Staphylococcus aureus - genetics
Staphylococcus aureus - physiology
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Summary:A multicopper oxidase gene from Staphylococcus aureus was cloned and overexpressed. Purified recombinant multicopper oxidase oxidized the substrate 3,3'-dimethoxybenzidine in the presence of copper. Disruption of mco showed copper sensitivity and H₂O₂ resistance, suggesting roles for mco in copper homeostasis and oxidative stress response. Northern blot analysis showed copper-induced mco transcription.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.9.5650-5653.2005