Maitotoxin-induced Free Ca Changes in Single Rat Hepatocytes

We report the results using bioluminescent and fluorescent indicators to investigate maitotoxin-induced free Ca changes in single rat hepatocytes. Maitotoxin generated a steadily rising free Ca increase after a long lag period. The free Ca increase was dependent on extracellular calcium and could be...

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Bibliographic Details
Published in:Cellular signalling 1999-11, Vol.11 (11), p.805-811
Main Authors: Woods, Niall M., Dixon, C.Jane, Yasumoto, Takeshi, Cuthbertson, K.S.Roy, Cobbold, Peter H.
Format: Article
Language:English
Subjects:
Adrenergic alpha-Agonists - pharmacology
Animals
Calcium - metabolism
Cells, Cultured
Free calcium oscillations
Liver - cytology
Liver - metabolism
Maitotoxin
Manganese
Marine Toxins - metabolism
Marine Toxins - pharmacology
Oxocins
Phenylephrine - pharmacology
Rat hepatocytes
Rats
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Summary:We report the results using bioluminescent and fluorescent indicators to investigate maitotoxin-induced free Ca changes in single rat hepatocytes. Maitotoxin generated a steadily rising free Ca increase after a long lag period. The free Ca increase was dependent on extracellular calcium and could be antagonised by chelation of extracellular calcium or the inclusion of nickel in the superfusate. Manganese-induced quench of cytoplasmic Fura2 dextran revealed an accelerated rate of calcium entry during the final period of the lag phase, immediately prior to the free Ca increase. Imaging experiments demonstrated a markedly different part of free Ca mobilisation compared with glycogenolytic stimuli. Moreover, the use of a combination of hormonal stimuli and maitotoxin revealed that some cells could exhibit free Ca oscillations despite steadily rising intracellular free Ca level. The significance of these observations in terms of the mechanism of action of maitotoxin and the mechanism of free Ca transient generation is discussed.
ISSN:0898-6568
1873-3913
DOI:10.1016/S0898-6568(99)00046-7