Biological sulphate reduction and redox mediator effects on azo dye decolourisation in anaerobic–aerobic sequencing batch reactors

• Published in: Enzyme and microbial technology 2005-04, Vol.36 (5), p.790-799
• Main Authors: Albuquerque, M.G.E., Lopes, A.T., Serralheiro, M.L., Novais, J.M., Pinheiro, H.M.
• Format: Article
• Language: English
• Subjects: Anaerobic-aerobic; Azo dyes; Biological and medical sciences; Biological treatment of waters; Biotechnology; Desulfovibrio alaskensis; Environment and pollution; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Redox mediator; Sequence batch reactors; Sulphate reduction
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/j.enzmictec.2005.01.005

In this work, the anaerobic period of an anaerobic–aerobic sequencing batch reactor was found to allow the reductive decolourisation of azo dyes. 1-l reactors were operated in 24-h cycles comprising anaerobic and aerobic reaction phases, fed with a simulated textile effluent including a reactive type (Remazol Brilliant Violet 5R) or an acid type (Acid Orange 7) azo dye. The aim was to assess the role of different redox phenomena in the anaerobic decolourisation process. Selective inhibition of sulphate reducing bacteria was carried out in the sulphate-containing, reactive dye fed reactor, resulting in nearly complete, though reversible and inhibition of decolourisation. The acid dye fed reactor's supplementation with sulphate, though resulting in sulphate reduction, did not improve decolourisation. Other redox mediators, namely quinones, were more effective in promoting electron transfer to the azo bond. Bio-augmentation of the acid dye fed reactor with a pure sulphate reducer strain known to decolourise azo dyes, Desulfovibrio alaskensis, was also carried out. Decolourisation was improved, but apparently as a result of the carbon source change required to support D. alaskensis growth. A chemically mediated reduction of the azo bond coupled to biological sulphate reduction, thus seemed to account for the high decolourisation yields of both dyes.