A new subtype of the metalloprotease toxin gene and the incidence of the three bft subtypes among Bacteroides fragilis isolates in Japan

The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft- 1 and bft- 2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bf...

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Published in:FEMS microbiology letters 2000, Vol.182 (1), p.171-176
Main Authors: Kato, Naoki, Liu, Cheng-Xu, Kato, Haru, Watanabe, Kunitomo, Tanaka, Yasunori, Yamamoto, Toshinobu, Suzuki, Kanzo, Ueno, Kazue
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Language:English
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Bacteroides fragilis
bft gene
Japan
Metalloprotease
Toxin gene
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container_issue 1
container_start_page 171
container_title FEMS microbiology letters
container_volume 182
creator Kato, Naoki
Liu, Cheng-Xu
Kato, Haru
Watanabe, Kunitomo
Tanaka, Yasunori
Yamamoto, Toshinobu
Suzuki, Kanzo
Ueno, Kazue
description The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft- 1 and bft- 2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bft- 1, and bft- 2 genes were determined for five, four, and four ETBF strains, respectively; the nucleotide sequence was identical among each bft subtype and the degree of identity between each subtype was between 89 and 94%. Most of the variations between the three subtypes were detected in the region encoding mature toxin. A multiplex PCR was developed with a four-primer mix to subtype the bft sequences. The subtyping of 143 ETBF isolates from extraintestinal and stool specimens of humans and cows showed that the bft- 1 was the most prevalent subtype, followed by bft- 2 and a new bft subtype. No other subtype was found among the strains tested.
doi_str_mv 10.1016/S0378-1097(99)00585-6
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subjects Bacteroides fragilis
bft gene
Japan
Metalloprotease
Toxin gene
title A new subtype of the metalloprotease toxin gene and the incidence of the three bft subtypes among Bacteroides fragilis isolates in Japan
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