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Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit

Plant pathogenic Pseudomonas syringae strains harbour a type III secretion pathway suggested to be involved in the delivery of effector proteins from the bacteria into plant cells. During plant interaction, the bacteria apparently produce surface appendages, termed Hrp pili, that are indispensable f...

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Published in:Molecular microbiology 1999-11, Vol.34 (4), p.736-744
Main Authors: Taira, S, Tuimala, J, Roine, E, Nurmiaho-Lassila, E.L, Savilahti, H, Romantschuk, M
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description Plant pathogenic Pseudomonas syringae strains harbour a type III secretion pathway suggested to be involved in the delivery of effector proteins from the bacteria into plant cells. During plant interaction, the bacteria apparently produce surface appendages, termed Hrp pili, that are indispensable for the secretion process. We have created an insertion mutation library, as well as deletion mutations to hrpA, the structural gene encoding Hrp pilin. Analysis of the mutants revealed gene regions important for hrpA expression, pilus assembly and pilus‐dependent autoagglutination of the bacteria. The majority of insertions in the amino‐terminal half of the pilin were tolerated without bacterial interaction with plants being affected, while the carboxy‐terminus appeared to be needed for pilus assembly. Insertions in the 5′ non‐translated region and the first codons within the open reading frame affected mRNA production or stability and abolished protein production.
doi_str_mv 10.1046/j.1365-2958.1999.01635.x
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source Wiley-Blackwell Read & Publish Collection
subjects 5' Untranslated Regions
bacterial proteins
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Base Sequence
DEAD-box RNA Helicases
deletions
DNA Mutational Analysis
DNA, Bacterial - analysis
Escherichia coli Proteins
Fimbriae Proteins
Fimbriae, Bacterial - genetics
Fimbriae, Bacterial - ultrastructure
Gene Deletion
Hrp protein
hrpA gene
insertional mutagenesis
Lycopersicon esculentum - microbiology
Membrane Proteins - genetics
Molecular Sequence Data
Mutagenesis, Insertional
mutation
Phenotype
pilin
plant pathogenic bacteria
protein secretion
Pseudomonas - genetics
Pseudomonas - metabolism
Pseudomonas syringae
RNA Helicases
RNA, Messenger - biosynthesis
title Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit
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