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Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit
Plant pathogenic Pseudomonas syringae strains harbour a type III secretion pathway suggested to be involved in the delivery of effector proteins from the bacteria into plant cells. During plant interaction, the bacteria apparently produce surface appendages, termed Hrp pili, that are indispensable f...
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Published in: | Molecular microbiology 1999-11, Vol.34 (4), p.736-744 |
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creator | Taira, S Tuimala, J Roine, E Nurmiaho-Lassila, E.L Savilahti, H Romantschuk, M |
description | Plant pathogenic Pseudomonas syringae strains harbour a type III secretion pathway suggested to be involved in the delivery of effector proteins from the bacteria into plant cells. During plant interaction, the bacteria apparently produce surface appendages, termed Hrp pili, that are indispensable for the secretion process. We have created an insertion mutation library, as well as deletion mutations to hrpA, the structural gene encoding Hrp pilin. Analysis of the mutants revealed gene regions important for hrpA expression, pilus assembly and pilus‐dependent autoagglutination of the bacteria. The majority of insertions in the amino‐terminal half of the pilin were tolerated without bacterial interaction with plants being affected, while the carboxy‐terminus appeared to be needed for pilus assembly. Insertions in the 5′ non‐translated region and the first codons within the open reading frame affected mRNA production or stability and abolished protein production. |
doi_str_mv | 10.1046/j.1365-2958.1999.01635.x |
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During plant interaction, the bacteria apparently produce surface appendages, termed Hrp pili, that are indispensable for the secretion process. We have created an insertion mutation library, as well as deletion mutations to hrpA, the structural gene encoding Hrp pilin. Analysis of the mutants revealed gene regions important for hrpA expression, pilus assembly and pilus‐dependent autoagglutination of the bacteria. The majority of insertions in the amino‐terminal half of the pilin were tolerated without bacterial interaction with plants being affected, while the carboxy‐terminus appeared to be needed for pilus assembly. 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During plant interaction, the bacteria apparently produce surface appendages, termed Hrp pili, that are indispensable for the secretion process. We have created an insertion mutation library, as well as deletion mutations to hrpA, the structural gene encoding Hrp pilin. Analysis of the mutants revealed gene regions important for hrpA expression, pilus assembly and pilus‐dependent autoagglutination of the bacteria. The majority of insertions in the amino‐terminal half of the pilin were tolerated without bacterial interaction with plants being affected, while the carboxy‐terminus appeared to be needed for pilus assembly. Insertions in the 5′ non‐translated region and the first codons within the open reading frame affected mRNA production or stability and abolished protein production.</description><subject>5' Untranslated Regions</subject><subject>bacterial proteins</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>DEAD-box RNA Helicases</subject><subject>deletions</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Bacterial - analysis</subject><subject>Escherichia coli Proteins</subject><subject>Fimbriae Proteins</subject><subject>Fimbriae, Bacterial - genetics</subject><subject>Fimbriae, Bacterial - ultrastructure</subject><subject>Gene Deletion</subject><subject>Hrp protein</subject><subject>hrpA gene</subject><subject>insertional mutagenesis</subject><subject>Lycopersicon esculentum - microbiology</subject><subject>Membrane Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>mutation</subject><subject>Phenotype</subject><subject>pilin</subject><subject>plant pathogenic bacteria</subject><subject>protein secretion</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - metabolism</subject><subject>Pseudomonas syringae</subject><subject>RNA Helicases</subject><subject>RNA, Messenger - biosynthesis</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqNkU9r2zAYh0XpWNJsX6HVqTd7-mPJ0qGHELo10LDBVthNSLacKNiWK9ld8u1n16XsuIsk-P2e94VHAECMUowy_uWYYspZQiQTKZZSpghzytLTBVi-B5dgiSRDCRXk9wJcxXhECFPE6UewwIjxjGG6BGo39Lp3vtU11ONxji5CX8H-YOGPaIfSN2MWYTwH1-61hd1LCnvf6N7DQ-jWcG9bC21b-HLM4UPoYOfqYQQGM7Su_wQ-VLqO9vPbvQJPX-9_bR6Sx-_ftpv1Y1IwzliCiSCsMJRVeZkxIzUSqJTG0CITJBNa57kx2FCCrCwI05k0pSBVJVFVclxyugK389wu-OfBxl41Lha2rnVr_RAVzjMseI7HopiLRfAxBlupLrhGh7PCSE1y1VFNDtXkUE1y1atcdRrR67cdg2ls-Q842xwLd3Phj6vt-b8Hq91uO71G_mbmK-2V3gcX1dNPMv0akRTRPKN_AYRskp0</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Taira, S</creator><creator>Tuimala, J</creator><creator>Roine, E</creator><creator>Nurmiaho-Lassila, E.L</creator><creator>Savilahti, H</creator><creator>Romantschuk, M</creator><general>Blackwell Science Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199911</creationdate><title>Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit</title><author>Taira, S ; Tuimala, J ; Roine, E ; Nurmiaho-Lassila, E.L ; Savilahti, H ; Romantschuk, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5655-12825cb35f7d45b9a080d9bb3c48248aa77bb1b320e9c25a49bd82ff90fd61d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>5' Untranslated Regions</topic><topic>bacterial proteins</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>DEAD-box RNA Helicases</topic><topic>deletions</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Bacterial - analysis</topic><topic>Escherichia coli Proteins</topic><topic>Fimbriae Proteins</topic><topic>Fimbriae, Bacterial - genetics</topic><topic>Fimbriae, Bacterial - ultrastructure</topic><topic>Gene Deletion</topic><topic>Hrp protein</topic><topic>hrpA gene</topic><topic>insertional mutagenesis</topic><topic>Lycopersicon esculentum - microbiology</topic><topic>Membrane Proteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>mutation</topic><topic>Phenotype</topic><topic>pilin</topic><topic>plant pathogenic bacteria</topic><topic>protein secretion</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - metabolism</topic><topic>Pseudomonas syringae</topic><topic>RNA Helicases</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taira, S</creatorcontrib><creatorcontrib>Tuimala, J</creatorcontrib><creatorcontrib>Roine, E</creatorcontrib><creatorcontrib>Nurmiaho-Lassila, E.L</creatorcontrib><creatorcontrib>Savilahti, H</creatorcontrib><creatorcontrib>Romantschuk, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taira, S</au><au>Tuimala, J</au><au>Roine, E</au><au>Nurmiaho-Lassila, E.L</au><au>Savilahti, H</au><au>Romantschuk, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1999-11</date><risdate>1999</risdate><volume>34</volume><issue>4</issue><spage>736</spage><epage>744</epage><pages>736-744</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Plant pathogenic Pseudomonas syringae strains harbour a type III secretion pathway suggested to be involved in the delivery of effector proteins from the bacteria into plant cells. During plant interaction, the bacteria apparently produce surface appendages, termed Hrp pili, that are indispensable for the secretion process. We have created an insertion mutation library, as well as deletion mutations to hrpA, the structural gene encoding Hrp pilin. Analysis of the mutants revealed gene regions important for hrpA expression, pilus assembly and pilus‐dependent autoagglutination of the bacteria. The majority of insertions in the amino‐terminal half of the pilin were tolerated without bacterial interaction with plants being affected, while the carboxy‐terminus appeared to be needed for pilus assembly. Insertions in the 5′ non‐translated region and the first codons within the open reading frame affected mRNA production or stability and abolished protein production.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>10564513</pmid><doi>10.1046/j.1365-2958.1999.01635.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 5' Untranslated Regions bacterial proteins Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Base Sequence DEAD-box RNA Helicases deletions DNA Mutational Analysis DNA, Bacterial - analysis Escherichia coli Proteins Fimbriae Proteins Fimbriae, Bacterial - genetics Fimbriae, Bacterial - ultrastructure Gene Deletion Hrp protein hrpA gene insertional mutagenesis Lycopersicon esculentum - microbiology Membrane Proteins - genetics Molecular Sequence Data Mutagenesis, Insertional mutation Phenotype pilin plant pathogenic bacteria protein secretion Pseudomonas - genetics Pseudomonas - metabolism Pseudomonas syringae RNA Helicases RNA, Messenger - biosynthesis |
title | Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit |
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