Hyaluronidase Expression in Human Skin Fibroblasts

Hyaluronidase activity has been detected for the first time in normal human dermal fibroblasts (HS27), as well as in fetal fibroblasts (FF24) and fibrosarcoma cells (HT1080). Enzymatic activity was secreted predominantly into the culture media, with minor amounts of activity associated with the cell...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-12, Vol.266 (1), p.268-273
Main Authors: Stair-Nawy, Susan, Csóka, Antonei Benjamin, Stern, Robert
Format: Article
Language:English
Subjects:
Cell Count
Cell Line
Culture Media, Conditioned - chemistry
Enzyme Activation - drug effects
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - enzymology
Fibrosarcoma - enzymology
Fibrosarcoma - pathology
Gene Expression - drug effects
Humans
Hyaluronoglucosaminidase - chemistry
Hyaluronoglucosaminidase - genetics
Hyaluronoglucosaminidase - metabolism
Hydrogen-Ion Concentration
Insulin - pharmacology
Interleukin-1 - pharmacology
Molecular Weight
Precipitin Tests
Reverse Transcriptase Polymerase Chain Reaction
Skin - cytology
Skin - embryology
Skin - enzymology
Tetradecanoylphorbol Acetate - pharmacology
Transforming Growth Factor beta - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hyaluronidase activity has been detected for the first time in normal human dermal fibroblasts (HS27), as well as in fetal fibroblasts (FF24) and fibrosarcoma cells (HT1080). Enzymatic activity was secreted predominantly into the culture media, with minor amounts of activity associated with the cell layer. In both classes of fibroblasts, hyaluronidase expression was confluence-dependent, with highest levels of activity occurring in quiescent, post-confluent cells. However, in the fibrosarcoma cell cultures, expression was independent of cell density. The enzyme had a pH optimum of 3.7 and on hyaluronan substrate gel zymography, activity occurred as a single band corresponding to an approximate molecular size of 57 kDa. The enzyme could be immunoprecipitated in its entirety using monoclonal antibodies raised against Hyal-1, human plasma hyaluronidase. PCR confirmed that fibroblast hyaluronidase was identical to Hyal-1. The conclusion by previous investigators using earlier technologies that fibroblasts do not contain hyaluronidase activity should be reevaluated.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.1802