A mutant of Chlamydomonas reinhardtii with reduced rate of photorespiration

Photorespiration rates under air-equilibrated conditions (0.04% CO2 and 21% O2) were measured in Chlamydomonas reinhardtii wild-type 2137, a phosphoglycolate-phosphatase-deficient (pgp1) mutant and a suppressor double mutant (7FR2N) derived from the pgp1 mutant. In both cells grown under 5% CO2 and...

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Bibliographic Details
Published in:Plant and cell physiology 1999-08, Vol.40 (8), p.792-799
Main Authors: Suzuki, K. (Tohoku National Agricultural Experiment Station, Morioka (Japan)), Mamedov, T.G, Ikawa, T
Format: Article
Language:English
Subjects:
5-bisphosphate carboxylase/oxygenase (Rubisco)
CHLAMYDOMONAS REINHARDTII
CO2concentrating mechanism (CCM)
Freshwater
Glycolate excretion
MUTANT
MUTANTES
MUTANTS
pgp1 gene
Phosphoglycolate phosphatase
Photorespiration
PHOTORESPIRATORY PATHWAY
Ribulose-1
VIA METABOLICA FOTORESPIRACION
VOIE METABOLISME PHOTORESPIRATOIRE
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Summary:Photorespiration rates under air-equilibrated conditions (0.04% CO2 and 21% O2) were measured in Chlamydomonas reinhardtii wild-type 2137, a phosphoglycolate-phosphatase-deficient (pgp1) mutant and a suppressor double mutant (7FR2N) derived from the pgp1 mutant. In both cells grown under 5% CO2 and adapted air for 24 h in the suppressor double mutant, the maximal rate of photorespiration (phosphoglycolate synthesis) was only about half of that in either the wild type or the pgp1 mutant (18-7F) cells. In the progeny, the reduced rate of photorespiration was accompanied by increased photosynthetic affinity for inorganic carbon and the capacity for growth under air whether accompanied by the pgp1 background or not. Tetrad analyses suggested that these three characteristics all resulted from a nuclear single-gene mutation at a site unlinked to the pgp1 mutation. The decrease in photorespiration was, however, not due to an increase in the CO2/O2 relative specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase of 7FR2N or of any other suppressor double mutants tested. The relationship between the decrease in the rate of photorespiration and the CO2-concentrating mechanism is discussed.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a029607