Sinorhizobium meliloti strain 1021 bioS and bdhA gene transcriptions are both affected by biotin available in defined medium

Sinorhizobium meliloti 1021 responds to external biotin signals from alfalfa plants through the bioS regulatory locus. Immunogold labeling and electron microscopy revealed that the BioS protein is located within the S. meliloti cytoplasm. Under biotin-limiting conditions the S. meliloti cell lumen w...

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Bibliographic Details
Published in:FEMS microbiology letters 2000, Vol.182 (1), p.41-44
Main Authors: Hofmann, Kai, Heinz, Elke B., Charles, Trevor C., Hoppert, Michael, Liebl, Wolfgang, Streit, Wolfgang R.
Format: Article
Language:English
Subjects:
Alfalfa
bdhA gene
bioS gene
Biotin
lacZ gene
poly- beta -hydroxybutyric acid
Polyhydroxybutyrate
Rhizosphere
Root colonization
Sinorhizobium meliloti
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Summary:Sinorhizobium meliloti 1021 responds to external biotin signals from alfalfa plants through the bioS regulatory locus. Immunogold labeling and electron microscopy revealed that the BioS protein is located within the S. meliloti cytoplasm. Under biotin-limiting conditions the S. meliloti cell lumen was filled with polyhydroxybutyrate (PHB) granules suggesting that either PHB synthesis or degradation are influenced by biotin. To test this hypothesis a 3-hydroxybutyrate-dehydrogenase- lacZ ( bdhA-lacZ) fusion was mobilized into S. meliloti. β-galactosidase tests revealed an overall 3.6–5.2-fold higher bdhA transcription in the presence of added biotin. Comparison of the bdhA and the bioS promoter regions identified several common motifs.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(99)00565-0