In vitro plant regeneration of Ilex paraguariensis (Aquifoliaceae)

Nodal segments as well as shoot tips and apical meristems of 2-yr-old "maté" plants (Ilex paraguariensis St. Hil.) were cultured in vitro to establish micropropagation systems. Maximum shoot regeneration was achieved when nodal segments were cultured with 1/4 Murashige and Skoog (MS) mediu...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1999-09, Vol.35 (5), p.401-402
Main Authors: Sansberro, P, Rey, H, Mroginski, L, Collavino, M
Format: Article
Language:English
Subjects:
Agroforestry
Apical meristems
Aquifoliaceae
Biological and medical sciences
Biotechnology
cell differentiation
culture media
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Ilex paraguariensis
In vitro propagation: entire plant regeneration from tissues and cell cultures
indole butyric acid
indole-3-butyric acid
Mate
Methods. Procedures. Technologies
Micropropagation
Plant cells and fungal cells
Plant growth regulators
Plants
Protocols and Research Reports
regenerative ability
roots
Shoot tip culture
shoots
sucrose
tissue culture
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Summary:Nodal segments as well as shoot tips and apical meristems of 2-yr-old "maté" plants (Ilex paraguariensis St. Hil.) were cultured in vitro to establish micropropagation systems. Maximum shoot regeneration was achieved when nodal segments were cultured with 1/4 Murashige and Skoog (MS) medium with 3% sucrose. We induced roots to differentiate by transferring the regenerated shoots onto the same medium, solidified with 2.5 g Phytagel per l and supplemented with indole-3-butyric acid (7.4 µM) and finally transferring shoots to 1/4 MS medium with 3% sucrose and lacking growth regulators. Plants were successfully established in soil.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-999-0054-5