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In vitro plant regeneration of Ilex paraguariensis (Aquifoliaceae)

Nodal segments as well as shoot tips and apical meristems of 2-yr-old "maté" plants (Ilex paraguariensis St. Hil.) were cultured in vitro to establish micropropagation systems. Maximum shoot regeneration was achieved when nodal segments were cultured with 1/4 Murashige and Skoog (MS) mediu...

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Published in:	In vitro cellular & developmental biology. Plant 1999-09, Vol.35 (5), p.401-402
Main Authors:	Sansberro, P, Rey, H, Mroginski, L, Collavino, M
Format:	Article
Language:	English
Subjects:	Agroforestry Apical meristems Aquifoliaceae Biological and medical sciences Biotechnology cell differentiation culture media Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Ilex paraguariensis In vitro propagation: entire plant regeneration from tissues and cell cultures indole butyric acid indole-3-butyric acid Mate Methods. Procedures. Technologies Micropropagation Plant cells and fungal cells Plant growth regulators Plants Protocols and Research Reports regenerative ability roots Shoot tip culture shoots sucrose tissue culture
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creator	Sansberro, P Rey, H Mroginski, L Collavino, M
description	Nodal segments as well as shoot tips and apical meristems of 2-yr-old "maté" plants (Ilex paraguariensis St. Hil.) were cultured in vitro to establish micropropagation systems. Maximum shoot regeneration was achieved when nodal segments were cultured with 1/4 Murashige and Skoog (MS) medium with 3% sucrose. We induced roots to differentiate by transferring the regenerated shoots onto the same medium, solidified with 2.5 g Phytagel per l and supplemented with indole-3-butyric acid (7.4 µM) and finally transferring shoots to 1/4 MS medium with 3% sucrose and lacking growth regulators. Plants were successfully established in soil.
doi_str_mv	10.1007/s11627-999-0054-5
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Technologies</topic><topic>Micropropagation</topic><topic>Plant cells and fungal cells</topic><topic>Plant growth regulators</topic><topic>Plants</topic><topic>Protocols and Research Reports</topic><topic>regenerative ability</topic><topic>roots</topic><topic>Shoot tip culture</topic><topic>shoots</topic><topic>sucrose</topic><topic>tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sansberro, P</creatorcontrib><creatorcontrib>Rey, H</creatorcontrib><creatorcontrib>Mroginski, L</creatorcontrib><creatorcontrib>Collavino, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>In vitro cellular & developmental biology. 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subjects	Agroforestry Apical meristems Aquifoliaceae Biological and medical sciences Biotechnology cell differentiation culture media Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Ilex paraguariensis In vitro propagation: entire plant regeneration from tissues and cell cultures indole butyric acid indole-3-butyric acid Mate Methods. Procedures. Technologies Micropropagation Plant cells and fungal cells Plant growth regulators Plants Protocols and Research Reports regenerative ability roots Shoot tip culture shoots sucrose tissue culture
title	In vitro plant regeneration of Ilex paraguariensis (Aquifoliaceae)
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