Effects of free amino acids on the isolated symbiotic algae of the coral Plesiastrea versipora (Lamarck): absence of a host release factor response

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 1998, Vol.120 (4), p.599-607
• Main Authors: Withers, Karen J.T, Grant, Adrienne J, Hinde, Rosalind
• Format: Article
• Language: English
• Subjects: Algae; Amino acids; Coral; Dinoflagellate; Host factor; Marine; Photosynthesis; Plesiastrea versipora; Symbiosis; Taurine
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/S1095-6433(98)10070-3

Symbiotic algae incubated in host tissue homogenate of the coral Plesiastrea versipora for 2 h in the light released at least four and a half times as much photosynthetically fixed carbon (range 13.8±3.1 to 158±9.5 nmol C/10 6 algae) as algae incubated in seawater (range 1.4±0.3 to 10.8±0.6 nmol C/10 6 algae) indicating the presence of ‘host release factor’. When algae were incubated in a low molecular weight fraction of homogenate containing partially purified ‘host release factor’ they also released more carbon (range 62.2±3.7 to 279±11.4 nmol C/10 6 algae) than algae incubated in seawater. This low molecular weight fraction contained free amino acids. We tested the hypothesis that the free amino acids in this fraction were responsible for ‘host release factor’ activity. Algae incubated in a mixture of free amino acids equivalent to those found in this fraction, released more fixed carbon (range 2.4±0.3 to 25.2±0.2 nmol C/10 6 algae) than algae incubated in seawater but in each experiment, release was much lower than when algae were incubated in host tissue homogenate. These data indicate that the stimulation of release of photosynthetically fixed carbon from the symbiotic algae of Plesiastrea versipora incubated in partially purified host release factor is not primarily due to the presence of free amino acids. We are continuing further studies to determine the exact nature of the active compound.