Analysis of Genetic Diversity in Rough Bluegrass Determined by RAPD Markers

Information on genetic variation in rough bluegrass (Poa trivialis L.), a cool‐season grass that is grown for sports fields and lawns, is needed. The objective of this study was to assess genetic variation in 27 accessions of rough bluegrass obtained from the National Plant Germplasm System (NPGS) b...

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Bibliographic Details
Published in:Crop science 2006-01, Vol.46 (1), p.162-167
Main Authors: Rajasekar, Shanmugam, Fei, Shui‐hang, Christians, Nick E.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Blue-grass
Correlation coefficient
Crop science
Deoxyribonucleic acid
DNA
Environmental aspects
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetic aspects
Genetic diversity
Genetic markers
Genetic resources, diversity
Genetics
Genetics and breeding of economic plants
Grasses
Plant material
Poa trivialis
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Summary:Information on genetic variation in rough bluegrass (Poa trivialis L.), a cool‐season grass that is grown for sports fields and lawns, is needed. The objective of this study was to assess genetic variation in 27 accessions of rough bluegrass obtained from the National Plant Germplasm System (NPGS) by random amplified polymorphic DNA (RAPD) markers. Fifteen of the 80 primers screened generated 64 highly repeatable polymorphic bands. A dendrogram constructed on the basis of the Unweighted Pair Group Method with Arithmetic Average (UPGMA) clustering algorithm revealed that 26 of 27 accessions formed two distinct clusters. Genetic similarity coefficients calculated from the RAPD data ranged from 0.07 to 0.74 with the lowest value of 0.07 measured between PI 254908, PI 594396, PI 250982, and PI 229782 from Iraq, the USA, Yugoslavia, and Iran, respectively. The highest value of 0.74 was measured between PI 225826, PI 289643, and PI 592521 from Denmark, Spain, and the USA, respectively. The cophenetic correlation coefficient (r) was 0.90, indicative of a very good fit between the data matrix and the resulting cluster analysis. Principal coordinate analysis (PCO) clearly grouped 26 accessions on the two axes, with a single accession that did not cluster with the others. The PCO clustering pattern corresponded well with the dendrogram. PI 254908 from Iraq did not cluster with any other accessions, either in the dendrogram or on the basis of the PCO.
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci2005.04-0008