Stimulated production of diosgenin in Dioscorea galeottiana cell suspension cultures by abiotic and biotic factors

Dioscorea deltoidea cell suspension cultures were established in modified Murashige and Skoog medium. The diosgenin production increased from 0.10 mg g-1 to 3.98 mg g-1 dry cell weight when cells were cultivated in the light and in a growth medium limited in phosphate and sucrose. The addition of 1....

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Bibliographic Details
Published in:Biotechnology letters 1999-10, Vol.21 (10), p.907-911
Main Authors: Rojas, R, Alba, J, Magana-Plaza, I, Cruz, F, Ramos-Valdivia, A.C
Format: Article
Language:English
Subjects:
Alternaria alternata
Alternaria tenuis
Biological and medical sciences
biological production
Biotechnology
Biotic factors
Cell culture
culture media
Dioscorea galeottiana
diosgenin
Establishment of new cell lines, improvement of cultural methods, mass culture
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
light
Methods. Procedures. Technologies
mycelium
Phosphates
Plant cells and fungal cells
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Summary:Dioscorea deltoidea cell suspension cultures were established in modified Murashige and Skoog medium. The diosgenin production increased from 0.10 mg g-1 to 3.98 mg g-1 dry cell weight when cells were cultivated in the light and in a growth medium limited in phosphate and sucrose. The addition of 1.3 g of autoclaved fungal mycelium of Alternaria tenuis per litre of cell culture growing in the dark induced the production of 0.04 mg diosgenin g-1 dry cell weight. In both cases, the production of diosgenin was preceded by a transient induction of isopentenyl diphosphate isomerase activity.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005598623728