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Stimulated production of diosgenin in Dioscorea galeottiana cell suspension cultures by abiotic and biotic factors
Dioscorea deltoidea cell suspension cultures were established in modified Murashige and Skoog medium. The diosgenin production increased from 0.10 mg g-1 to 3.98 mg g-1 dry cell weight when cells were cultivated in the light and in a growth medium limited in phosphate and sucrose. The addition of 1....
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| Published in: | Biotechnology letters 1999-10, Vol.21 (10), p.907-911 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c242t-d17546a05b5080f44c26e7e04df2dac19408ef2e612ed5f7c50278df6ad9ccd23 |
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| container_end_page | 911 |
| container_issue | 10 |
| container_start_page | 907 |
| container_title | Biotechnology letters |
| container_volume | 21 |
| creator | Rojas, R Alba, J Magana-Plaza, I Cruz, F Ramos-Valdivia, A.C |
| description | Dioscorea deltoidea cell suspension cultures were established in modified Murashige and Skoog medium. The diosgenin production increased from 0.10 mg g-1 to 3.98 mg g-1 dry cell weight when cells were cultivated in the light and in a growth medium limited in phosphate and sucrose. The addition of 1.3 g of autoclaved fungal mycelium of Alternaria tenuis per litre of cell culture growing in the dark induced the production of 0.04 mg diosgenin g-1 dry cell weight. In both cases, the production of diosgenin was preceded by a transient induction of isopentenyl diphosphate isomerase activity. |
| doi_str_mv | 10.1023/A:1005598623728 |
| format | article |
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The diosgenin production increased from 0.10 mg g-1 to 3.98 mg g-1 dry cell weight when cells were cultivated in the light and in a growth medium limited in phosphate and sucrose. The addition of 1.3 g of autoclaved fungal mycelium of Alternaria tenuis per litre of cell culture growing in the dark induced the production of 0.04 mg diosgenin g-1 dry cell weight. In both cases, the production of diosgenin was preceded by a transient induction of isopentenyl diphosphate isomerase activity.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/A:1005598623728</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Alternaria alternata ; Alternaria tenuis ; Biological and medical sciences ; biological production ; Biotechnology ; Biotic factors ; Cell culture ; culture media ; Dioscorea galeottiana ; diosgenin ; Establishment of new cell lines, improvement of cultural methods, mass culture ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; light ; Methods. Procedures. 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The diosgenin production increased from 0.10 mg g-1 to 3.98 mg g-1 dry cell weight when cells were cultivated in the light and in a growth medium limited in phosphate and sucrose. The addition of 1.3 g of autoclaved fungal mycelium of Alternaria tenuis per litre of cell culture growing in the dark induced the production of 0.04 mg diosgenin g-1 dry cell weight. In both cases, the production of diosgenin was preceded by a transient induction of isopentenyl diphosphate isomerase activity.</description><subject>Alternaria alternata</subject><subject>Alternaria tenuis</subject><subject>Biological and medical sciences</subject><subject>biological production</subject><subject>Biotechnology</subject><subject>Biotic factors</subject><subject>Cell culture</subject><subject>culture media</subject><subject>Dioscorea galeottiana</subject><subject>diosgenin</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass culture</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>light</subject><subject>Methods. Procedures. 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| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 1999-10, Vol.21 (10), p.907-911 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17442005 |
| source | Springer Link |
| subjects | Alternaria alternata Alternaria tenuis Biological and medical sciences biological production Biotechnology Biotic factors Cell culture culture media Dioscorea galeottiana diosgenin Establishment of new cell lines, improvement of cultural methods, mass culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology light Methods. Procedures. Technologies mycelium Phosphates Plant cells and fungal cells |
| title | Stimulated production of diosgenin in Dioscorea galeottiana cell suspension cultures by abiotic and biotic factors |
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