Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus

An obligate alkalophilic Bacillus sphaericus strain, isolated from alkaline soils in the Himalaya, produced an extracellular protease which was optimally active at 50-55 °C and pH 10.5. The enzyme was stable in presence of 500 mg chlorine l^sup -1^ and as a detergent additive. Its stability in prese...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 1999-10, Vol.21 (10), p.921-924
Main Authors: SINGH, J, VOHRA, R. M, SAHOO, D. K
Format: Article
Language:English
Subjects:
alkaline proteinase
Alkaline soils
Alkalinity
Bacillus sphaericus
Bacteria
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Chlorine
Detergents
Fundamental and applied biological sciences. Psychology
gelatin
Miscellaneous
Mission oriented research
Proteases
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An obligate alkalophilic Bacillus sphaericus strain, isolated from alkaline soils in the Himalaya, produced an extracellular protease which was optimally active at 50-55 °C and pH 10.5. The enzyme was stable in presence of 500 mg chlorine l^sup -1^ and as a detergent additive. Its stability in presence of laundry detergents was comparable to that of commercial proteases. The gelatin layer in 25 g of used X-ray films was efficiently hydrolyzed within 12 min at 50 °C, pH 11.0 and 25 U protease/ml.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005502824637