A Rapid and Quantitative Detection of Cellular Protein Kinase Activity Based on MALDI-TOF-MS

A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly comp...

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Bibliographic Details
Published in:Chemistry letters 2014-05, Vol.43 (5), p.658-660
Main Authors: Otsubo, Yuki, Ikeda, Hiromu, Kamimoto, Junpei, Niidome, Takuro, Mori, Takeshi, Katayama, Yoshiki
Format: Article
Language:English
Subjects:
Cellular
Drugs
Isotopes
Kinases
Labels
Marking
Peptides
Proteins
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Summary:A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.131227