Convenient detection of metal-DNA, metal-RNA, and metal-protein adducts with a click-modified Pt(II) complex

cis-[Pt(2-azido-1,3-propanediamine)Cl2] is a reagent for high-yield post-treatment fluorescent labelling of Pt(II) biomolecular targets using click chemistry and exhibits a bias in conformational isomers in the context of duplex DNA. Pt-protein adducts are detected using BSA as a model. Following in...

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Bibliographic Details
Published in:Dalton transactions : an international journal of inorganic chemistry 2015-02, Vol.44 (8), p.3536-3539
Main Authors: Moghaddam, Alan D, White, Jonathan D, Cunningham, Rachael M, Loes, Andrea N, Haley, Michael M, DeRose, Victoria J
Format: Article
Language:English
Subjects:
Adducts
Animals
Bias
Cattle
Click Chemistry
Coordination Complexes - chemical synthesis
Coordination Complexes - chemistry
Deoxyribonucleic acid
DNA - chemistry
DNA Adducts - chemistry
Fluorescence
Hydrazines - chemistry
Isomers
Labelling
Platinum
Platinum - chemistry
Proteins - chemistry
Proteins - metabolism
Ribonucleic acids
RNA - chemistry
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - metabolism
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Summary:cis-[Pt(2-azido-1,3-propanediamine)Cl2] is a reagent for high-yield post-treatment fluorescent labelling of Pt(II) biomolecular targets using click chemistry and exhibits a bias in conformational isomers in the context of duplex DNA. Pt-protein adducts are detected using BSA as a model. Following in vivo treatment, long-lived Pt-RNA adducts are detected on ribosomal RNA.
ISSN:1477-9226
1477-9234
DOI:10.1039/c4dt02649g