Using cytochrome P4501A1 expression in liver and blubber to understand effects of persistent organic pollutant exposure in stranded Pacific Island cetaceans

Elevated levels of persistent organic pollutants (POPs) have been reported in tropical Pacific Island cetaceans and their environment. In addition, recent health concerns in cetacean populations have warranted investigation into potential physiological effects from POP exposure for this region. Cyto...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 2015-09, Vol.34 (9), p.1989-1995
Main Authors: Bachman, Melannie J., Foltz, Kerry M., Lynch, Jennifer M., West, Kristi L., Jensen, Brenda A.
Format: Article
Language:English
Subjects:
Adipose Tissue - drug effects
Adipose Tissue - metabolism
Animals
Biomarker
Biomarkers
Categories
Cetacea
Cetacea - metabolism
Cetaceans
Correlation
Cytochrome
Cytochrome P-450 CYP1A1 - metabolism
Cytochrome P450
Dolphins & porpoises
Effects
Elevated
Exposure
Gene expression
Hawaii
Immunoblotting
Immunohistochemistry
Islands
Liver
Liver - drug effects
Liver - metabolism
Organic Chemicals - chemistry
Organic Chemicals - toxicity
Pacific Islands
Persistent organic pollutants
Pollutants
Stenella
Up-Regulation - drug effects
Water Pollutants, Chemical - chemistry
Water Pollutants, Chemical - toxicity
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Summary:Elevated levels of persistent organic pollutants (POPs) have been reported in tropical Pacific Island cetaceans and their environment. In addition, recent health concerns in cetacean populations have warranted investigation into potential physiological effects from POP exposure for this region. Cytochrome P450 1A1 (CYP1A1) is a candidate for examining such effects. This well‐studied biomarker of exposure and effect was examined in stranded cetacean liver using immunoblot (n = 39, 16 species) and blubber using immunohistochemistry (n = 23, 10 species). Paired tissue samples allowed for CYP1A1 comparisons not only between species but also within each individual animal to examine differences between tissue types. Liver CYP1A1 expression correlated positively and significantly with blubber concentrations of all POP categories (n = 39, p < 0.050) except octachlorostyrene and pentachlorobenzene (p > 0.100). Among Stenella species, liver CYP1A1 tissue expression was correlated negatively with the sum of all blubber layer endothelial cell CYP1A1 expression (n = 14, p = 0.049). Overall, elevated expression of liver CYP1A1 confirms its use as a biomarker of POP exposure to cetaceans stranded in the tropical Pacific basin. Environ Toxicol Chem 2015;34:1989–1995. © 2015 SETAC
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.3018