Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin

A molecularly imprinted polymer film was in situ polymerized on a carboxyl functionalized multi-walled carbon nanotubes modified glassy carbon electrode surface under room temperature. This technique provides a promising imprinting approach for protein in an aqueous solution using 3-(3-aminopropyl)-...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-12, Vol.74, p.792-798
Main Authors: Wang, Yanying, Han, Miao, Liu, Guishen, Hou, Xiaodong, Huang, Yina, Wu, Kangbing, Li, Chunya
Format: Article
Language:English
Subjects:
Animals
Biosensing Techniques
Bovine serum albumin
Cattle
Detection
Electrochemical sensing
Electrochemical Techniques
Electrodes
In-situ-polymerization
Ionic liquid
Ionic Liquids
Limit of Detection
Milk
Molecular Imprinting
Molecularly imprinted
Nanotubes, Carbon - chemistry
Polymerization
Recognition
Sensors
Serum albumin
Serum Albumin, Bovine - isolation & purification
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Summary:A molecularly imprinted polymer film was in situ polymerized on a carboxyl functionalized multi-walled carbon nanotubes modified glassy carbon electrode surface under room temperature. This technique provides a promising imprinting approach for protein in an aqueous solution using 3-(3-aminopropyl)-1-vinylimidazolium tetrafluoroborate ionic liquid as functional monomer, N, N′-methylenebisacrylamide as crossing linker, ammonium persulfate and N,N,N′,N′-tetramethylethylenediamine as initiator, and bovine serum albumin (BSA) as template. The molecularly imprinted polymerized ionic liquid film shows enhanced accessibility, high specificity and sensitivity towards BSA. Electrochemical sensing performance of the imprinted sensor was thoroughly investigated using K3Fe[CN]6/K4Fe[CN]6 as electroactive probes. Under optimal conditions, the current difference before and after specific recognition of BSA was found linearly related to its concentration in the range from 1.50×10−9 to 1.50×10−6molL−1. The detection limit was calculated to be 3.91×10−10molL−1 (S/N=3). The practical application of the imprinted sensor was demonstrated by determining BSA in liquid milk samples. •Facile fabrication of BSA imprinted polymerized ionic liquid film electrode.•Morphological and voltammetric characterization of the BSA imprinted film electrode.•Enhanced selectivity towards BSA were demonstrated.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2015.07.046