Double clicking for site-specific coupling of multiple enzymes

A method to site-specifically couple multiple enzymes is reported. The approach is based on the site-specific incorporation of a clickable non-natural amino acid into enzymes and two compatible click reactions. The multi-enzyme reaction system exhibited enhanced catalytic efficiency over the respect...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2015-09, Vol.51 (71), p.13607-13610
Main Authors: Lim, Sung In, Cho, Jinhwan, Kwon, Inchan
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - chemistry
Amino Acids - metabolism
Azides - chemistry
Biological Assay
Catalysis
Catalysts
Click Chemistry
Compatibility
Enzymes
Joining
Molecular Structure
Multienzyme Complexes - chemistry
Multienzyme Complexes - metabolism
Substrate Specificity
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Summary:A method to site-specifically couple multiple enzymes is reported. The approach is based on the site-specific incorporation of a clickable non-natural amino acid into enzymes and two compatible click reactions. The multi-enzyme reaction system exhibited enhanced catalytic efficiency over the respective free enzymes.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc04611d