Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases—application to feed supplements

In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in su...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2012-08, Vol.404 (3), p.799-808
Main Authors: Neubauer, Stefan, Rugova, Ariana, Chu, Dinh Binh, Drexler, Hedda, Ganner, Anja, Sauer, Michael, Mattanovich, Diethard, Hann, Stephan, Koellensperger, Gunda
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Animal Feed - analysis
Biochemistry
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography
Chromatography, Reverse-Phase
Complex Mixtures - chemistry
Food Science
Laboratory Medicine
Limit of Detection
Mass spectrometry
Mathematical analysis
Monitoring/Environmental Analysis
Nucleosides
Nucleosides - analysis
Nucleotides
Nucleotides - analysis
Original Paper
Phosphorus - analysis
Purines - analysis
Pyrimidines - analysis
Separation
Speciation
Tandem Mass Spectrometry
Yeast
Yeasts - chemistry
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Summary:In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP–LC–MS–MS, excellent limits of detection
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-012-6170-9