A biomimetic sensor surface to detect anti- beta 2-glycoprotein-I antibodies as a marker for antiphospholipid syndrome

A biomimetic sensor has been developed, that allows for quantification of autoantibodies related to the antiphospholipid syndrome (APS). Autoantibodies directed against the beta 2-glycoprotein-I ( beta 2GP-I) are known as the best markers for diagnosis of APS, however, detection of such antibodies i...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2012-05, Vol.403 (3), p.713-717
Main Authors: Hilbig, Urs, Bleher, Oliver, Le Blanc, Alexander, Gauglitz, Gunter
Format: Article
Language:English
Subjects:
Antibodies
Calibration
Chips
Markers
Mathematical analysis
Membranes
Sensors
Stripping
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Summary:A biomimetic sensor has been developed, that allows for quantification of autoantibodies related to the antiphospholipid syndrome (APS). Autoantibodies directed against the beta 2-glycoprotein-I ( beta 2GP-I) are known as the best markers for diagnosis of APS, however, detection of such antibodies is still a challenge. The epitopes of beta 2GP-I are exposed upon binding to negatively charged membranes. The surface of the sensor chips was therefore modified with such type of membranes, on which beta 2GP-I molecules were subsequently immobilized as recognition elements. Using the label-free method, reflectometric interference spectroscopy, it was possible to quantify anti- beta 2GP-I antibodies and to calibrate the sensor chip in buffer. A mild regeneration procedure allows for many consecutive measurements without stripping off the membrane in between.
ISSN:1618-2642
DOI:10.1007/s00216-012-5831-z